miR-146a在口腔鳞癌组织及细胞中的表达及功能研究

Effect and Functioanl Role of miR-146a in Oral Squamous Cell Carcinoma Tissues and Cell Lines

目的:探究miR-146a在口腔鳞状细胞癌(OSCC)组织及细胞中的表达及其对口腔鳞癌细胞生物学行为能力的影响。方法:采用荧光定量聚合酶链反应(RT-PCR)法检测OSCC组织及细胞中miR-146a的表达,观察miR-146a对OSCC细胞增殖、侵袭、迁移等能力的影响。结果:与癌旁组织相比OSCC组织中miR-146a呈明显高表达(P<0.05);与正常口腔上皮细胞HOK相比,OSCC细胞系Scc9、Scc25、Cal27中miR-146a表达水平明显偏高(P<0.05)。上调miR-146a表达后,Scc9及Cal27的细胞增殖能力明显增加(P<0.05),Scc9、Scc25、Cal27的侵袭和迁移能力明显增加(P<0.05);抑制miR-146a表达后,Scc9、Scc25、Cal27的侵袭和迁移能力明显降低(P<0.05)。结论:miR-146a在OSCC和口腔癌细胞中呈明显高表达,对于促进口腔癌的增殖、侵袭和迁移具有重要意义。

Objective：To explore the expression and its functional role of miR-146 ain oral squamous cell carcinoma（OSCC）tissues.Methods：RT-PCR was performed to detect the expression level of miR-146 ain OSCC tissues and OSCC cell lines.CCK8 assay,transwell assay and wound healing assay were performed to observer cell proliferation,invasion and migration abilities.Results：The expression of miR-146 awas significantly higher in OSCC tissues compared to the adjacent tissues（P0.05）,and the expression of miR-146 ain OSCC cell lines such as Scc9,Scc25,and Cal27 were substantially higher than the normal oral epithelial cells（HOK）（P0.05）.The proliferation,invasion and migration abilities were substantially enhanced in Scc9 and Cal27 cells transfected with miR-146 amimics（P0.05）.The invasion and migration abilities were substantially decreased in Scc9,Scc25,and Cal27 cells transfected with miR-146 ainhibitor（P0.05）,but the proliferation abilities showed no statistically diffierents in Scc9,Scc25,and Cal27 cells transfected with miR-146 ainhibitor.Conclusion：miR-146 ashows a significant expression in OSCC tissues and cell lines,is correlated with proliferation,invasion and migration of OSCC.