镰形棘豆黄酮苷元对免疫抑制小鼠细胞因子的影响

The Effects of Flavonoid Glycosides Extracted from LianXing JiDou on the Cytokines of the Immunosuppressive Mice

目的:研究藏药镰形棘豆黄酮苷元对免疫抑制小鼠细胞因子的影响。方法:将60只昆明种小鼠随机分为6组:空白组,模型组,镰形棘豆黄酮苷元高、中、低剂量组(336、168、84 mg/kg)和盐酸左旋咪唑组(25 mg/kg),每组10只。镰形棘豆黄酮苷元高、中、低剂量组分别灌胃不同剂量的黄酮苷元;盐酸左旋咪唑组灌胃左旋咪唑25 mg/kg;空白组和模型组灌服等体积5%吐温-80溶液,共10天。第8天开始注射环磷酰胺制备免疫低下小鼠模型,除空白组腹腔注射生理盐水外,其他各组分别腹腔注射环磷酰胺,共3天。采用酶免仪分别测定血清中白细胞介素1、2、4、8、10(IL-1、IL-2、IL-4、IL-8、IL-10),肿瘤坏死因子α(TNF-α),干扰素γ(IFN-γ)的含量及脾淋巴细胞培养上清液中IL-2、IL-4、IL-10、TNF-α、IFN-γ的含量,观察镰形棘豆黄酮苷元对免疫抑制小鼠细胞因子的影响。结果:与模型组比较,镰形棘豆黄酮苷元各剂量组能有效升高免疫抑制小鼠血清和脾淋巴细胞培养液中各细胞因子的含量,差异有统计学意义(P<0.05)。结论:镰形棘豆黄酮苷元能够拮抗环磷酰胺所致小鼠的免疫抑制作用,增强小鼠的免疫功能。

Objective： To explore the influence of flavonoid glycosides extracted from Tibetan medicine LianXing JiDou （Oxytropis falcata Bunge） on the cytokines of the immunosuppressive mice. Methods： Sixty mice were randomized into six groups： the blank group, the model group, high, moderate and low dose groups of flavonoid glycosides extracted from LianXing JiDou （336, 168, 84 mg/kg） as well as levamisole hydrochloride group （25 mg/kg）, ten mice each group. High, moderate and low dose groups of flavonoid glycosides extracted from LianXing JiDou accepted intragastric administration of flavonoid glycosides in different doses respectively; the blank group and the model group were drenched with the same amount of 5% tween-80 solutions, for ten days. The immunosuppressive mice models were prepared by injecting cyclophosphamide since the eighth day, except the blank group received peritoneal injection of physiological saline, other groups accepted peritoneal injection of cyclophosphamide for three days. The contents of IL-1, IL-2, IL-4, IL-8, IL-10, TNF-α and IFN-γ in the serum and the contents of IL-2, IL-4, IL-10, TNF-α and IFN-γ in the supernatant of cultured splenic lymphocytes were detected by using ELISA method, to observe the influence of flavonoid glycosides extracted from Tibetan medicine LianXing JiDou on the cytokines of the immunosuppressive mice. Results： Compared with the model group, different doses groups of flavonoid glycosides extracted from LianXing JiDou could effectively raise the contents of cytokines in blood serum and the supernatant of cultured splenic lymphocytes of the immunosuppressive mice, and the difference had statistical meaning （P〈0.05）. Conclusion： Flavonoid glycosides extracted from LianXing JiDou could resist cyclophosphamide-induced immunosuppressive action of the mice, and improve the immunologic function of the mice.