胶质瘤中LC3B与CD68^+小胶质细胞、CD4^+和CD8^+T淋巴细胞的相关性及意义

Correlation of LC3B with CD68^+ microglia,CD4^+T lymphocytes,and CD8^+T lymphocytes in gliomas and its significance

目的研究不同级别胶质瘤组织中自噬相关蛋白LC3B的表达与CD68^+小胶质细胞、CD4^+和CD8^+T淋巴细胞数量的相关性并探讨其意义。方法采用免疫组化、Western blot检测127例不同级别胶质瘤及40例瘤旁正常组织中LC3B的表达,免疫组化检测CD68^+小胶质细胞、CD4^+和CD8^+T淋巴细胞的数量,分析其相关性及意义。结果 (1)免疫组化及Western blot检测结果显示LC3B的表达在瘤旁正常组织、低级别胶质瘤及高级别胶质瘤中呈不同程度的升高,且组间比较差异具有显著性(P<0.05)。(2)CD68^+小胶质细胞、CD4^+和CD8^+T淋巴细胞在胶质瘤中的数量明显高于瘤旁正常组织(P<0.05),且高级别胶质瘤明显高于低级别胶质瘤(P<0.05)。(3)LC3B的表达与CD68^+小胶质细胞、CD4^+和CD8^+T淋巴细胞的数量在不同级别胶质瘤中均呈正相关,低级别胶质瘤中相关系数分别为0.466、0.599、0.537,高级别胶质瘤中相关系数分别为0.657、0.608、0.561。(4)胶质瘤中LC3B的表达及CD68^+小胶质细胞、CD4^+和CD8^+T淋巴细胞的数量均与肿瘤的大小有关(P<0.05)。结论在胶质瘤组织中LC3B、CD68^+小胶质细胞、CD4^+和CD8^+T淋巴细胞均呈高表达,且LC3B的表达与CD68^+小胶质细胞、CD4^+和CD8^+T淋巴细胞的数量呈正相关,LC3B可能是影响胶质瘤细胞免疫的因素之一。

Objective To examine the correlation of the expression of microtubule-associated protein light chain 3B （LC3B） with the numbers of CD68 ＋ microglia,CD4 ＋ T lymphocytes,and CD8 ＋ T lymphocytes in different grades of gliomas and to investigate its significance.Methods LC3B expression in 127 gliomas tissues of different grades and 40 tumor-adjacent normal brain tissues was determined using immunohistochemistry （IHC） and Western blot.The numbers of CD68 ＋ microglia,CD4 ＋ T lymphocytes,and CD8 ＋ T lymphocytes in these tissues were measured using IHC.The correlation between LC3B and the immune cells,as well as its significance,was analyzed.Results （1） IHC and Western blot showed that LC3B expression was significantly higher in low-grade gliomas than in tumor-adjacent normal brain tissues,and significantly higher in high-grade gliomas than in low-grade gliomas （all P ＜ 0.05）.（2） The numbers of CD68＋ microglia,CD4 ＋ T lymphocytes,and CD8 ＋ T lymphocytes were significantly higher in gliomas than in tumor-adjacent normal brain tissue （P ＜ 0.05）,and significantly higher in high-grade gliomas than in low-grade gliomas （P ＜ 0.05）.（3） LC3 B expression was positively correlated with the numbers of CD68 ＋ microglia,CD4 ＋ T lymphocytes,and CD8 ＋ T lymphocytes in low-grade and high-grade gliomas （low-grade gliomas：r =0.466,0.599,and 0.537,all P ＜ 0.05;high-grade gliomas：r =0.657,0.608,and 0.561,all P ＜ 0.05）.（4） LC3B expression and the numbers of CD68 ＋ microglia,CD4 ＋ T lymphocytes,and CD8 ＋ T lymphocytes in gliomas were significantly correlated with tumor size （P ＜ 0.05）.Conclusions LC3B expression is positively correlated with the numbers of CD68 ＋ microglia,CD4 ＋ T lymphocytes,and CD8 ＋ T lymphocytes in glioma tissues,and LC3B may be one of the factors that affect the cellular immunity of glioma patients.