糖尿病型白内障患者血清和房水中部分抗氧化指标及血管内皮生长因子水平

Partial Antioxidant Indexes and the Level of Vascular Endothelial Growth Factor in Serum and Aqueous Humor of Patients with Diabetic Cataract

目的:探讨糖尿病性白内障(DC)患者血清和房水中抗氧化指标及血管内皮生长因子(VEGF)的变化。方法:70例DC患者作为观察组(初始期17例、肿胀期15例、成熟期25例及过熟期13例),另选取同期轻度眼外伤患者30例为对照组,观察组于入院时、对照组于伤后2 h内采集血液及房水标本进行检测,采用ELISA法测定人过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)及VEGF水平,比色法测定总抗氧化能力(TAC)、维生素E(Vit E)含量,比较两组受试者、各分期观察组患者血清和房水中CAT、TAC、GSH-Px、Vit E及VEGF变化。结果:观察组患者随着病程的进展,初始期、肿胀期、成熟期及过熟期血清及房水中CAT、TAC、GSH-Px、Vit E水平逐渐降低(P<0.05),且明显低于对照组(P<0.05);观察组患者随着病程的进展,初始期、肿胀期、成熟期、过熟期血清和房水VEGF水平逐渐升高(P<0.05),观察组肿胀期、成熟期、过熟期血清和房水VEGF水平高于对照组(P<0.05),而初始期与对照组比较差异无统计学意义(P>0.05)。结论:DC的发生发展与机体氧化应激有关,VEGF参与DC患者视网膜病变的发病过程。

Objective： To explore the changes of antioxidant indexes and vascular endothelial growth factor（ VEGF） in the serum and aqueous humor of patients with diabetic cataract（ DC）. Methods：70 patients with DC were treated as the observation group（ 17 cases of initial stage,15 cases of swelling stage,mature 25 cases and 13 cases over mature stage）. 30 cases of patients with mild ocular trauma in the same period were selected as the control group. The observation group at the time of admission as well as the blood and water samples of the control group was collected at 2 h after injury by ELISA method（ CAT） determinating catalase,glutathione peroxidase（ GSH-Px） and the level of VEGF. The total antioxidant capacity was determined by colorimetric method（ TAC）,and vitamin E（ Vit E） were compared between the two groups of subjects and each stage of the observation group in serum and aqueous humor of patients with the change of CAT,TAC,GSH-Px,Vit,E,VEGF. Results： In the observation group,the levels of CAT,TAC,GSH-Px and Vit E decreased gradually with the progression of the disease,the initial stage,the swelling stage,the mature stage and the over mature stage（ P〈0. 05）,and it was significantly lower than the control group（ P〈0. 05）. In the observation group,the serum and aqueous VEGF levels increased gradually with the progression of the disease,the initial stage,the swelling phase,the mature stage and the over mature stage（ P〈0. 05）.The levels of serum and aqueous VEGF in the observation group,swelling phase,mature stage and over mature stage were higher than those in the control group（ P〈0. 05）,but there was statistically no significant difference between the initial stage and the control group（ P〈0. 05）. Conclusions： The occurrence and development of DC are related to oxidative stress in organism. VEGF is involved in the pathogenesis of retinopathy in DC patients.