NRG1转染对骨髓基质干细胞表达VEGF的影响

Influence of NRG1 transfection on expression of VEGF in bone marrow stromal stem cells

目的探讨神经调节因子1(neuregulin-1,NRG1)转染是否可以促进骨髓基质干细胞(bone marrow stromal cells,BMSCs)表达血管内皮生长因子(vascular endothelial growth factor,VEGF)。方法提取与培养3只SD大鼠的BMSCs,采用NRG1质粒转染BMSCs并提取上清液,采用ELISA法测定转染组上清液中VEGF的含量,将非转染BMSCs的样本作为对照组,采用免疫组化方法检测细胞VEGF的表达。结果 BMSCs多数细胞处于伸展状态,呈梭形;经ELISA方法检测,转染组上清液VEGF的含量随时间增加呈增长趋势,与对照组比较,差异有统计学意义(P<0.05);经免疫组化检测,转染组细胞VEGF阳性率为91.07%,对照组细胞阳性率为70.82%,差异有统计学意义(P<0.05)。结论 NRG1转染可以促进BMSCs表达VEGF。

Objective To explore whether bone marrow stromal stem ceils （BMSCs） could been promoted by NRG1 transfection to VEGF expression.Methods The BMSCs of three SD rats were isolated and cultured,BMSCs were transfeeted with NRG1 plasmids,and the supernates were extracted.The contents of VEGF in the supernates were detected by the method of ELISA,and the samples of BMSCs were regarded as the control group.The expression of VEGF in BMSCs was detected by immunohistochemical method.Results Most of BMSCs were stretched and spindle-shaped.The levels of VEGF in the transfection group were increased with time by ELISA detection,compared with the control group,there was significant difference （P〈0.05）.The VEGF positve cell rate of the transfection group was 91.07% by immunohistochemi- cal detection,while the positive cell rate of the control group was 70.82%,there was significant difference compared with the control group （P〈0.05）.Coneluslon BMSCs can promoted by NRG1 transfection to express VEGF.