肺癌患者胸腔积液中UHRF1 mRNA的表达和临床意义

Expression and clinical significance of UHRF1 mRNA in pleural effusion of patients with lung cancer

目的探讨肺癌患者胸腔积液中脱落细胞的泛素样含PHD和环指域1(UHRF1)mRNA表达水平及临床诊断价值。方法选取2015年10月至2017年2月南京医科大学附属江宁医院收治的胸腔积液患者124例,根据病理学诊断结果分为恶性胸腔积液组(60例)和良性胸腔积液组(64例),采用实时荧光定量聚合酶链反应技术检测两组UHRF1mRNA表达水平,分别与胸腔积液中脱落细胞学检查结果、癌胚抗原(CEA)和DNA异倍体细胞检测结果进行比较,并分析其与临床病理因素的关系。结果恶性胸腔积液组中UHRF1mRNA相对表达量为(4.53±4.33),明显高于良性胸腔积液组(1.61±1.56),差异有统计学意义(t=5.057,P=0.000)。受试者工作特征曲线分析显示,UHRF1mRNA、CEA单独检测及联合检测的曲线下面积分别为0.753、0.879、0.861,灵敏度分别为53.48%、78.18%、74.42%,特异度分别为88.24%、93.44%、86.27%。在不同年龄、性别、肿瘤定位、组织学分型、CEA水平及有无吸烟史患者的胸腔积液中,UHRF1mRNA相对表达量差异无统计学意义(P>0.05);脱落细胞阳性和异倍体细胞阳性患者胸腔积液中UHRF1mRNA相对表达量高于阴性患者,差异有统计学意义(P<0.05)。结论UHRF1mRNA对恶性胸腔积液具有较高的临床诊断价值。

Objective To investigate the expression levels and clinical diagnostic value of ubiquitin-like with PHD and ring finger domains 1（UHRF1）mRNA in exfoliated cells of pleural effusion in the patients with lung cancer.Methods A total of 124 patients with pleural effusion in Jiangning Hospital affiliated to Nanjing Medical University from October 2015 to February 2017 were selected and divided into the malignant pleural effusion group（60 cases）and the benign pleural effusion group（64 cases）according to the pathological diagnosis results.The expression levels of UHRF1 mRNA in the two groups were detected by real-time quantitative polymerase chain reaction,and their detection results were compared with the those of exfoliative cytology,carcinoembryonic antigen（CEA）and DNA aneuploid cell in pleural effusion.Their relationship with clinical pathological factors was analyzed.Results The relative expression level of UHRF1 mRNA in the malignant pleural effusion group was（4.53±4.33）,which was significantly higher than（1.61＋1.56）in the benign pleural effusion group,the difference was statistically significant（t=5.057,P=0.000）.The receiver operating characteristic（ROC）curve analysis showed that the areas under the curve of UHRF1 mRNA,CEA single detection and combined detection were 0.753,0.879,0.861,and the sensitivities were 53.48%,78.18%,74.42%,and the specificities were 88.24%,93.44%,86.27% respectively.In different age,gender,tumor location,histological type,CEA level and smoking history in patients with pleural effusion,there was no significant difference in the relative expression of UHRF1 mRNA（P0.05）;the relative expression of UHRF1 and mRNA in pleural effusion positive cells and aneuploid cells positive patients was higher than that in negative patients,and the difference was statistically significant（P0.05）.Conclusion UHRF1 mRNA has high clinical diagnostic value in malignant pleural effusion.