ERα基因PvuⅡ、XbaⅠ和ERβ基因RsaⅠ、AluⅠ酶切多态性与冠心病的相关性研究

Study on correlation between ERαgene PvuⅡ,XbaⅠ and ERβgene RsaⅠ,AluⅠ digestion polymorphism with coronary heart disease

目的研究盐城地区雌激素受体α(ERα)基因PvuⅡ、XbaⅠ和雌激素受体β(ERβ)RsaⅠ、AluⅠ酶切多态性与冠状动脉粥样硬化性心脏病(CHD)危险因素的相关性。方法选取124例CHD患者和163例健康体检者分别作为CHD组和对照组,采用酶法测定三酰甘油(TG)和总胆固醇(TC),采用直接法测定高密度脂蛋白(HDL)和低密度脂蛋白(LDL),采用聚合酶链反应-限制片断长度多态性方法分析两组ERα基因PvuⅡ、XbaⅠ和ERβ基因RsaⅠ、AluⅠ酶切多态性。结果 CHD组有吸烟史、有家族史、合并高血压及糖尿病的比率,以及体质量指数、TC、TG、LDL-C水平均明显高于对照组,差异有统计学意义(P<0.05);男女之间各指标差异无统计学意义(P>0.05)。两组ERα基因PvuⅡ、XbaⅠ和ERβ基因RsaⅠ、AluⅠ基因频率分布与理论分布无差别,均符合Hardy-Weinberg遗传平衡,具有群体代表性。CHD组中,pp、xx、RR、AA基因型最多,PP、XX、rr、aa基因型最少;对照组中,Pp、xx、RR、AA基因型最多,PP、XX、rr、aa基因型最少。CHD组p、x基因分布频率明显高于对照组,差异有统计学意义(P<0.05)。结论雌激素基因多态性可能是一个有效的治疗CHD的靶点,p、x基因分布频率可能与CHD危险因素相关。

Objective To investigate the correlation of ERαgene PvuⅡ,XbaⅠ and ERβgene RsaⅠ,AluⅠ digestion polymorphism with coronary atherosclerotic heart disease（CHD）risk factors in Yancheng area.Methods A total of 124 cases of CHD and163 persons undergoing physical examination served as the CHD group and CON group.The enzyme method was adopted to detect TG and TC.The direction method was adopted to detect HDL and LDL.ERαgene PvuⅡ,XbaⅠand ERβgene RsaⅠ,AluⅠ digestion polymorphisms were detected by adopting RFLP-PCR.Results The ratios of smoking history,family history,complicating hypertension and diabetes,and the level of body mass index,TC,TG and LDL-C in the CHD group were significantly higher than those in the control group,the difference was statistically significant（P0.05）.The various indicators had no statistically difference between male and female（P0.05）.The frequency distribution and geographic distribution of ERαgene PvuⅡ,XbaⅠ and ERβgene RsaⅠ,AluⅠdigestion polymorphisms had no difference between the two groups,all conformed to Hardy-Weinberg genetic equilibrium and had the group representativeness.pp,xx,RR and AA genotypes in the CHD group were maximal,while PP,XX,rr and aa genotypes were minimal;Pp,xx,RR and AA genotypes in the CON group were maximal,while PP,XX,rr and aa genotypes were minimal.The distribution frequency of p and x genes in the CHD group was significantly higher than that in the control group,the difference was statistically significant（P0.05）.Conclusion The estrogen gene polymorphism might be a target spot for effectively treating CHD,and p and x gene distribution frequency may be related with CHD risk factors.