用新鲜血校准血细胞分析仪及结果比对的探讨

Calibration of blood cell analyzer and the results comparing with new blood

目的:用新鲜血对不同型号的血细胞分析仪进行校准和比对。方法:定期校准一台性能优良、技术较先进的贝克曼LH780血细胞分析仪(简称LH780),做为参比仪器,然后用健康者新鲜全血对LH780做重复性试验,计算新鲜全血在待校准仪器上测得的各项均值及其与校准靶值的偏差,再用健康者新鲜全血去校准其它血细胞分析仪。并以LH780评价其他4台血细胞分析仪可比性和相关性。结果:LH780新鲜血做精密度测试,其WBC、RBC、HGB、PLT检测CV值均小于2%,符合CLIA’88《1988年的美国临床实验室改进规范》。其它四部血细胞分析仪(包括LH750、XE-5000-1、XE-5000-2、XE-800i)检测结果与LH780相比对,其结果密切相关,具有可比性。结论:用新鲜血对不同型号血细胞分析仪进行定期核准和比对,既能保证各仪器结果间的精密度及准确度,又降低了成本。

Objective： To use the new blood for different types of blood cells analyzer calibration and comparison.Methods：regular calibration of a good performance, more advanced technology of beckman LH780 blood cell analyzer （ hereinafter referred to as LH780） , as a reference instrument, and then use the healthy fresh whole blood of LH780 repeatability test, the calculation of fresh whole blood in measured the average stay calibration instrument and its calibration and the deviation of the target value with a healthy fresh whole blood to calibrate other blood cell analyzer. And with other four LH780 evaluation blood cell analyzer, comparability and correlation.Results：LH780 new blood do precision test, the WBC, RBC, HGB, PLT detection CV values are less than 2%, conform to the CLIA 88＂standard of clinical laboratory improvement of the United States in 1988＂ Other four blood cell analyzer （ including LH750, XE-5000-1, XE-5000-2, XE-800 I） test results compared with LH780 right, closely related to the result, comparable.Conclusion：with the new blood in different type of blood cell analyzer for approval on a regular basis and than, can not only ensure that the results of each instrument between the precision and accuracy, and reduce the cost