九里香叶对去卵巢大鼠体积骨密度、血钙、血磷、碱性磷酸酶、骨组织形态学的影响

Effects of Murraya Paniculat Leaf on Volumetric Bone Mineral Density,Blood calcium,Blood Phosphorus,Alkaline phosphatase,bone Histomorphology in Ovari-ectomized Rats

目的:观察九里香叶对去卵巢大鼠体积骨密度、血钙、血磷、碱性磷酸酶、骨组织形态学的影响。方法:取60只SPF级SD雌性大鼠,通过切除双侧卵巢复制骨质疏松模型。术后7 d分组给药。模型组:生理盐水(0.4 mg/kg·d);对照组:生理盐水(0.4 mg/kg·d);戊酸雌二醇组:0.4 m g/kg·d;九里香高剂量组:200 mg/kg·d;九里香中剂量组:100 mg/kg·d;九里香低剂量组:50 mg/kg·d。连续给药90 d。给药结束后,测定大鼠体积骨密度,采用电极法测定血钙;速率法测定血磷、碱性磷酸酶。大鼠右侧股骨HE染色,计量骨小梁平均宽度、骨小梁面积百分率。结果:与对照组比较,模型组大鼠体积骨密度明显降低,差异有统计学意义(P<0.05);模型组大鼠血钙、碱性磷酸酶明显降低,差异有统计学意义(P<0.05),血磷无差异;模型组大鼠骨小梁平均宽度及骨小梁面积百分率明显降低,差异有统计学意义。与模型组比较,九里香叶高剂量组大鼠体积骨密度明显增高,差异有统计学意义(P<0.05);九里香叶高剂量组大鼠血钙明显增高,差异有统计学意义(P<0.05),血磷无统计学意义;九里香叶低剂量组大鼠碱性磷酸酶明显降低,差异有统计学意义(P<0.05);九里香叶高剂量组骨小梁平均宽度及骨小梁面积百分率明显增高,差异有统计学意义(P<0.05)。结论:九里香叶影响去卵巢大鼠骨质疏松体积骨密度、血钙、碱性磷酸酶、骨小梁平均宽度及骨小梁面积百分率变化,具有抗骨质疏松作用。

Objective： To observe the effect of Murraya Paniculat leaf on volumetric bone mineral density,blood calcium,blood phosphorus,Alkaline phosphatase,bone histomorphology in ovari-ectomized rats. Methods： A total of 60 SPF SD female rats were taken as models by removing bilateral ovaries. After 7 days of operation,all rats were divided into six groups： model group（ normal saline,0. 4 mg/kg·d）,control operation group（ normal saline,0. 4 mg/kg·d）,Estradiol Valerate group（ 0. 4 mg/kg·d）,Murraya Paniculat leaf high dose group（ 200 mg/kg·d）,Murraya Paniculat leaf middle dose group（ 100 mg/kg·d） and Murraya Paniculat leaf low dose group（ 50 mg/kg·d）. Drugs were given once a day for 90 days. Using electrode analysis to measure volumetric bone mineral density of rats and velocity method to measure blood calcium and blood phosphorus and alkaline phosphatase. Use HE staining on the right side of the femur of rats,then measured the average width and trabecular bone area percentage.Results： Compared with the control group,volumetric bone mineral density of model group rats were reduced（ P〈0. 05）,blood phosphorus and Alkaline phosphatase of model group rats were lower,and the difference is significant（ P〈0. 05）,also,the average width and trabecular bone area percentage of model group rats were lower（ P〈0. 05）. No difference was found in blood phosphorus. Compared with model group,volumetric bone mineral density and blood calcium and the average width and trabecular bone area percentage were higher in Murraya Paniculat leaf high dose group（ P〈0. 05）. No difference was found in blood phosphorus.Also,alkaline phosphatase in Murraya Paniculat leaf low dose group was lower with significant difference（ P〈0. 05）. Conclusion： Murraya Paniculat leaf could influence volumetric bone mineral density,blood calcium,blood phosphorus,alkaline phosphatase,the average width and trabecular bone area percentage in ovari-ectomized rats. It has anti-osteoporosis effect.