摘要
目的人参鹿茸药对配伍对睾丸间质细胞(TM_3)细胞增殖活性的比较。方法运用均匀设计考察的方法,制备不同比例人参鹿茸药对配伍样品,应用配伍的样品干预TM_3细胞,MTT法检测细胞增殖率,并对TM_3细胞增殖作用进行评价。结果人参鹿茸配伍的各组样品均具促进TM_3细胞增殖的作用,人参与鹿茸按2.1:1剂量配伍时该药对促进TM_3细胞增殖活性的作用最佳。结论人参鹿茸药对不同剂量配伍时对促进TM_3细胞增殖活性具有协同作用,为人参鹿茸药对在补气壮阳方面的作用提供了有力的科学依据。
Objective To compare the effect of Panax Ginseng-Hairy Antler compatibility on TM_3 cell proliferation.Methods Uniform design method was used to prepare compatible samples of different proportions of Panax GinsengHairy Antler compatibility.TM_3 cells were intervened by compatible samples,cell proliferation rate was detected by MTT method,and the proliferation of TM_3 cells was evaluated.Results All samples of Panax Ginseng-Hairy Antler pairs promoted the role of TM_3 cell proliferation,and there was a best effect on promoting the proliferation when the combination of Panax Ginseng and Hairy Antler according to the dosage of 2.1:1.Conclusion Panax GinsengHairy Antler pairs has synergistic effect on proliferation of TM_3 cell in different dose compatibility,and it provides a powerful scientific basis for the effect of Panax Ginseng-Hairy Antler compatibilty on invigorating qi and tonifying yang.
出处
《吉林中医药》
2017年第11期1140-1142,共3页
Jilin Journal of Chinese Medicine
基金
国家自然基金课题(81373936)
国家自然基金课题(31570347)
吉林省卫生和计划生育委员会科研计划课题(2015Z067)
吉林省科技发展计划项目(20140203015 YY)
关键词
人参
鹿茸
MTT法
TM3细胞
补气壮阳
Ginseng
Pilose Antler
MTT assay
TM3 cell
invigorating qi and tonifying yang