摘要
目的探讨顺铂联合乌司他丁处理胃癌细胞BGC-823后,观察细胞增殖率和细胞周期相关蛋白的变化。方法通过体外培养胃癌细胞BGC-823。实验分为四组:对照组、顺铂化疗组、乌司他丁组以及顺铂+乌司他丁组。MTF法检测BGC-823细胞增殖率,观察顺铂及乌司他丁对胃癌细胞系增殖率的影响。WesternBlot法检测胃癌细胞中细胞周期相关蛋白CyclinG1和MEDF2D的表达,RT—PCR法检测细胞中CyclinG1的表达水平。结果细胞增殖率结果表明:与对照组相比,顺铂组、乌司他丁组和顺铂+乌司他丁组的细胞增殖率明显降低,有显著的统计学意义(P〈0.01);与顺铂组和乌司他丁组相比,顺铂+乌司他丁组的细胞增殖率明显降低,有统计学意义(P〈0.01)。WesternBlot结果显示:与对照组相比,顺铂组和乌司他丁组的CyclinG1和MEDF2D蛋白降低(P〈0.05),顺铂+乌司他丁组的CyclinG1和MEDF2D蛋白亦明显降低,有显著的统计学意义(P〈0.01);与顺铂组相比,顺铂+乌司他丁组的CyclinG1和MEDF2D蛋白明显降低,有统计学意义(P〈0.01)。RT—PCR结果表明:与对照组相比,顺铂组和乌司他丁组的CyclinG1的mRNA水平明显降低;与顺铂组和乌司他丁组相比,顺铂+乌司他丁组的CyclinGI的mRNA水平显著降低(P〈0.01)。结论乌司他丁能降低经顺铂化疗患者的胃癌细胞BGC-823的增殖率,这可能是通过降低细胞周期相关蛋白CyclinG1和MEDF2D的表达所致。
Objective To study the changes of the proliferation and Cyelin protein after the managemeng of the gastric cancer cell BGC - 823 treated by the Cisplatin combined with Ulinastatin. Methods The gastric cancer cell BGC - 823 was cultured in vitro and divided into four groups: the control group, Cisplatin group, Ulinastatin group and Cisplatin + Ulinastatin group. The gastric cancer cell and the proliferation were detected by MTT; The eyelin protein of Cyelin GI and MEDF2D was detected by Western Blot and the level of miRNA Cyelin G1 was detected by RT - PCT. Results MTT showed that compared with control group, the pro- liferation rate was obviously decreased in Cisplatin group, Ulinastatin group and Cisplatin + Ulinastatin group. There was significant difference ( P 〈 0. 01 ). Compared with Cisplatin group and Ulinastatin group, the proliferation rate was obviously decreased in Cispl- atin + Ulinastatin group. There was statiseally difference ( P 〈 0. 01 ). Western Blot showed that compared with the control group, the protein of Cyelin G1 and MEDF2D decreased in Cisplatin group and Ulinastatin group ( P 〈 0. 05 ) , and in Cisplatin + Ulinastatin group the protein also obviously decreased (P 〈 0. 01 ). Compared with Cisplatin group and Ulinastatin group, the levels of Cyclin G1 and MEDF2D were significantly decreased (P 〈 0.01 ). RT -PCR results showed that compared with the control group, the level of miRNA Cyclin G1 was decreased in Cisplatin group, Ulinastatin group and Cisplatin + Ulinastatin group. Compared with Cisplatin group and Ulinastatin group, the level of miRNA Cyclin G1 was decreased in Cisplatin + Ulinastatin group (P 〈 0. 05). Conclusion Ulinastatin can decrease the proliferation of gastric cancer cell BGC - 823 and is relate to the decreasing the cyclin protein of Cyclin G1 and MEDF2D.
出处
《国际老年医学杂志》
2017年第6期274-277,共4页
International Journal of Geriatrics
关键词
胃癌
乌司他丁
顺铂
增殖
细胞周期蛋白
Gastric Cancer
Ulinastatin
Cisplatin
Proliferation
Cyclin Protein