年龄对老年人血清胃蛋白酶原Ⅰ、胃蛋白酶原Ⅱ和胃泌素-17结果判读的影响

Influence of age on interpretation of serum pepsinogen Ⅰ,pepsinogen Ⅱ and gastrin-17 in elderly

目的探讨年龄对血清胃蛋白酶原Ⅰ(PGⅠ)、胃蛋白酶原Ⅱ(PGⅡ)和胃泌素-17(G-17)结果判读的可能影响。方法收集2016年在南京医科大学第一附属医院进行健康体检的居民血清标本共2732份。采用酶联免疫吸附法对血清PGⅠ、PGⅡ和G-17水平进行检测,并计算PGⅠ和PGⅡ的比值(PGR)。依据年龄、性别和试剂盒参考值范围,将所有结果按照检测项目分为正常组(所有指标均正常)和异常组(有1项及以上指标异常)以及老年亚组和非老年亚组。应用ln转换,分别采用独立样本t检验和线性相关分析对数据进行统计。结果(1)正常组中,老年亚组血清PGⅠ和PGⅡ水平均高于非老年亚组,血清G-17水平低于非老年亚组。异常组中,老年亚组血清PGⅠ、PGⅡ和G-17水平均高于非老年亚组,而PGR显著低于非老年亚组,差异有统计学意义。(2)进一步按性别分层分析,正常组中,老年亚组男性血清PGⅠ和PGⅡ水平高于同组女性以及非老年亚组的男性;老年亚组女性G-17水平低于非老年亚组女性;非老年亚组男性PGⅠ水平高于同组女性。异常组中,老年亚组4项指标在性别分布中差异均无统计学意义,非老年亚组男性PGⅠ、PGⅡ水平高于女性,G-17水平低于女性;非老年亚组女性PGⅠ、PGⅡ水平低于老年亚组女性,PGR水平高于老年亚组女性;非老年亚组男性PGR水平高于老年亚组男性,而G-17水平低于老年亚组男性。结论年龄和性别是影响老年人PGⅠ、PGⅡ和胃泌素-17检测结果的因素。临床工作中需要考虑这两个因素以降低老年人群的假阳性率。

Objective To investigate the impact of age on the levels of serum pepsinogen Ⅰ（ PGⅠ）,pepsinogen Ⅱ（ PGⅡ）,and gastrin-17（ G-17）. Methods We collected 2732 serum samples of residents who received health examination in the First Affiliated Hospital of Nanjing Medical University in2016. Serum levels of PG Ⅰ,PG Ⅱ,and G-17 were detected by enzyme linked immunosorbent assay,and then the ratio of PGⅠ to PGⅡ（ PGR） was calculated. According to age,gender,and the reference ranges of the detection kits,all data were divided into normal group and abnormal group,elderly sub group and non-elderly sub group. Independent-sample t test,linear correlation analysis were used for statistical analysis. Results（ 1） In normal group,serum PG Ⅰ and PG Ⅱ levels in elderly subgroup were higher than those in non-elderly group,and serum G-17 level was lower than that in non-elderly subgroup. In abnormal group, serum PG Ⅰ, PG Ⅱ, and G-17 levels in elderly subgroup were significantly higher than those in non-elderly subgroup,and PGR was significantly lower than that in nonelderly subgroup.（ 2） In normal group,serum PGⅠ and PGⅡ levels of male in elderly subgroup were higher than those of the female in elderly subgroup and male of non-elderly subgroup; G-17 level of female in elderly subgroup was significantly lower than that in non-elderly subgroup; PGⅠ level of nonelderly subgroup in male was higher than that in female. In normal group,there were no significant difference between different genders in elderly subgroup. Serum PGⅠ and PGⅡ levels of male in nonelderly subgroup were higher than those of female,and G-17 level were significantly lower than that of female; Serum PG Ⅰ and PG Ⅱ levels of female in non-elderly subgroup were lower than those of the female in elderly subgroup; PGR level was significantly higher than that of female in elderly subgroup;PGR level of male in non-elderly subgroup was significantly higher,and G-17 level was lower than that of male in elderly subgroup. Conclusions Age and gender are influencing factors of serum PGⅠ,PGⅡ,and G-17. Both should be concerned in clinical work to decrease false positive rate of the elderly.