NO对抗精子抗体阳性大鼠精子自发顶体反应的影响

Effect of NO on the Spontaneous Acrosome Reaction in AsAb Positive Rat Spermatozoa

目的 :探讨 NO对抗精子抗体 ( As Ab)阳性大鼠精子自发顶体反应的影响。方法 :采用主动免疫法建立 As Ab阳性大鼠动物模型 ;浅盘凝集实验和 ELISA法检测大鼠血清 As Ab;考马斯亮蓝染色法进行精子顶体染色 ,以观察大鼠自发精子顶体反应率。结果 :As Ab阳性大鼠精子自发 AR%下降 ,且内源性 NO含量、精子内 SOD和 Na+ -K+ ATP酶活性明显低于对照组 ;低剂量 NO( SNP1 0 - 9～ 1 0 - 8mol/L)可提高 As Ab阳性大鼠精子自发顶体反应率、SOD活性 ,但对 Na+ -K+ATP酶活性无影响 ( P>0 .0 5 )。高剂量 NO( SNP1 0 - 6 ～ 1 0 - 4mol/L)则进一步降低上述三项指标。结论 :As Ab阳性大鼠自发顶体反应率降低可能与精子内 NO生成减少、O2 - ·产生增多 ( SOD活性降低 )有关。低浓度 NO可以灭活过量的超氧化物而提高 As Ab阳性大鼠精子自发顶体反应率 ;但过高浓度

Objectives:To explore the effect of NO on the spontaneous acrosome r eaction in antisperm antibody (AsAb) positive rat spermatozoa.Method s:The rat model of AsAb was set up by artificial immunity. The level of AsAb in blood serum was determined by TAT and ELISA. Rat spermatozoa was vis ualized by staining acrosome with the dye Coomassie brilliant blue. NO concentra tion in rat spermatozoa was assayed by HPLC. Results:The percentage of acrosome reaction, NO concentration,SOD and Na +-K +ATPase act ivity in AsAb positive rat spermatozoa significantly decreased as compared with the control group. Low dose of NO (SNP10 -9 ～10 -8 mol/L) increase the percentage of acrosome reaction and SOD activity,but had no effect on Na +-K +ATPase activity.High dose of NO(SNP10 -6 ～10 -4 mol/L) decreased th e three iterms.Conclusion:The decrease of percentage of acrosome reaction in positive AsAb rat spermatozoa might be related to decrease of NO and increase of O 2 -·(SOD activity decreased) in sperm.The low dose o f NO might increase the percentage of acrosome reaction in AsAb positive rat by inhibiting of the superoxide,while high dose of NO was harmful to sperm functio ns.