外周血miR-145表达在食管癌放疗近期疗效评价中的意义

Significance of miR-145 expression in peripheral blood in short-term efficacy evaluation of radiotherapy for esophageal carcinoma

目的探讨食管癌患者外周血微小RNA·145（miR-145）表达水平与临床病理特征的关系，及其在放疗近期疗效评价中的意义。方法收集在青岛大学附属医院确诊的单纯行根治放疗的食管癌患者52例，采用反转录聚合酶链反应（RT-PCR）方法检测入组患者放疗前血浆miR-145的相对表达量，并分析其与基本临床病理特征、放疗近期疗效的相关性。结果在52例食管癌患者中，TNM分期Ⅰ～Ⅱ期患者外周血miR-145的相对表达量M（QR）为3．14（1．44），高于Ⅲ～Ⅳ期患者的1．71（0．48），差异具有统计学意义（Z=-3．002，P=0．003），而外周血miR-145的相对表达量与患者的年龄（Z=-0．403，P=0．687）、性别（Z=-1．179，P=0．238）、饮酒史（Z=-0．389，P=0．697）无明显相关性。放疗近期疗效评价为完全缓解（CR）、部分缓解（PR）和无缓解（NC）组患者外周血miR-145的相对表达量分别为3．27（1．64）、1．83（1．26）和1．40（2．51），差异具有统计学意义（H=6．567，P=0．038）。结论外周血miR-145表达水平与食管癌临床分期有显著相关性，其表达水平与食管癌放疗的近期疗效呈正相关，表达量高提示食管癌放疗的近期疗效较好。

Objective To investigate the relationship between tho expression level of microRNA-145 （miR-145） in peripheral blood and clinicopathological characteristics of esophageal cancer patients and eva- luate its significance in short-term efficacy of radiotherapy Methods A total of 52 patients with esophageal cancer diagnosed in Affiliated Hospital of Qingdao University were collected, and all of the patients received ra- diotherapy alone. The relative expression level of miR-145 in peripheral blood was detected by using the method of reverse transcriptase polymerase chain reaction （RT-PCR） before radiotherapy~ The correlations between the expression of miR-145 and pathological characteristics as well as short-term efficacy of radiotherapy were ana- lyzed. Results In the 52 patients, the relative expression of miR-145 [ M（ QR ） ] in peripheral blood of clini- cal stage I - II was 3.14 （ 1.44）, higher than that of stage m - 1v [ 1.71 （ 0. 48 ） ], and the difference was statistically significant （ Z = - 3. 002, P = 0.003 ）. The expression level of miR-145 was not related with the age （Z = - 0. 403, P = 0. 687）, gender （ Z = - 1. 179, P = 0. 238） and history of drinking （ Z = - 0. 389, P = 0.697 ）. The expression levels of miR-i45 in complete response （CR） , partial response （PR） and no change （NC） groups were 3.27 （ 1.64）, 1.83 （ 1.26 ） and 1.40 （ 2.51 ）, respectively, and the difference was statistically significant （ H = 6. 567, P = 0.038 ）. Conclusion The expression level of miR-145 in peri- pheral blood is significantly correlated with the clinical stage of esophageal cancer. Its expression level is posi- tively correlated with the short-term efficacy of esophageal cancer radiotherapy. High expression of miR-145 shows a better short-term efficacy of radiotherapy.