不同管径注射针对脂肪细胞活性的影响

Effects of different diameter injection needles on the activity of adipocytes

目的检测不同管径注射针注射的脂肪细胞活性,研究注射针管径对脂肪细胞活性的影响。方法采用传统负压抽吸方式获得脂肪颗粒悬液200 ml;经双层纱布过滤并漂洗后,转入20支5 ml注射器,随机分为4组,每组5支注射器,其中1组为对照组。余3组以5 ml注射器连接不同管径注射针(12 G、16 G、18 G)匀速推出脂肪颗粒,每组分别注入5个15 ml离心管中。以Calcein-AM/Hoechst33342荧光染色法检测对照组及注射出的脂肪细胞活性,计算细胞成活率,比较注射针管径大小对脂肪细胞活性的影响。结果经12 G、16 G、18 G注射针注射过程顺利,无明显阻力。通过Calcein-AM/Hoechst33342染色法检测经不同管径注射针注射的脂肪颗粒中细胞成活率,对照组、12 G、16 G、18 G注射针管组细胞成活率分别为(83.53±7.92)%、(82.62±8.09)%、(75.78±10.94)%、(68.45±11.24)%,注射针管各组间差异具有统计学意义(P<0.05),12 G注射针管组细胞成活率与对照组差异无统计学意义(P>0.05)。结论单位时间内注射一定量的脂肪颗粒随着注射针管内径减小,脂肪颗粒细胞成活比将随之降低,增大注射针管径可增高注射后细胞成活比。

Objective To detect the activity of adipocytes of injection needles of different diameters and to study the effects of injection needles with different diameters on the activity of adipocytes. Methods Two hundred milliliters of fat granules obtained by traditional negative pressure suction were prepared. After filtration and rinse with double layer gauze, the fat granules were then transferred into20 syringes（5 ml） and randomly divided into 4 groups（n=5）. One was the control group. In the other three groups, different diameter injection needles（12 G、16 G、and 18 G） were employed using 5 ml syringes to separate the fat granules, then fat granules were transferred into centrifuge tubes（15 ml） at a constant rate. Adipocyte activity was detected in the control group and the other three groups with Calcein-AM/Hoechst 33342 fluorescence staining. The rate of viable cells was calculated and the results of the use of different diameters of injection needles on the activity of adipocytes were compared. Results The injection needle（12 G、16 G、18 G） process was smooth without obvious resistance. The viability of fat particles injected in the control group and the groups with the three different diameter injection needles（12 G、16 G、18 G） detected by Calcein-AM/Hoechst 33342 fluorescence stain was（83.53±7.92）%,（82.62±8.09）%,（75.78±10.94）%, and（68.45±11.24）%, the difference among the three groups（12 G、16 G、18 G） was statistically significant（P〈0.05）, but was not statistically significant between the control group and the 12 G group（P〈0.05）. Conclusion Different diameters of injection needles have differing impacts on the activity of fat adipocyte. By decreasing the diameter of the injection syringe, live cells are reduced in fat particles when a certain amount of fat particles per unit of time is injected. Increasing the diameter of the needle can increase the ratio of live cells after injection.