人巨细胞病毒核酸标准物质研制与应用

The development and application of reference materials for human cytomegalovirus nucleic acids

人巨细胞病毒(human cytomegalovirus,HCMV)是威胁人类健康的最重要病原之一。随着分子生物学技术的发展,HCMV核酸水平的检测逐渐得到了广泛的认可,这也就需要研制HCMV核酸标准物质来保证检验数据的真实可靠。本文简要介绍了国内外目前存在的HCMV核酸标准物质的研制情况,并对这些标准物质在应用中遇到的问题进行了阐述,旨在为人巨细胞病毒核酸检测诊断试剂的标准化,以及为其质量控制提供参考。

Human cytomegalovirus（HCMV） is one of the most important pathogens which are threatening people health in the world. With the development of molecular biology techniques, value of HCMV DNA detection has been recognized gradually. The reference materials for HCMV test are critical to guarantee the quality of the test. In this study, the research progress of the reference materials for HCMV nucleic acid amplification testing and the problems in application will be summarized. The aim of this study was to standardize the diagnostic reagents for human cytomegalovirus nucleic acid testing and provide a reference for the quality control.