酶传感器中GOD酶固定化方法条件的选择

Choice of fixation method of GOD in the enzyme biosensor

目的 研究尼龙网上胆碱氧化酶的不同固定化方法对酶活性的影响 ,以及测定体系pH值的选择。方法 采用活化载体 共价结合法和戊二醛交联法。结果 发现用改进后的活化载体 共价结合法固定的酶活性高 ,使用寿命长 ,酶不易损失 ,重现性好。

Objective To study the effect to enzyme activity using different method to fix enzyme's on the nylon net and choice system pH of measurement buffer.Methods Using the activate carry- covalence combinative methods and glutaraldehyde methods.Results The enzyme in the improvement system had more activity and good reappearance serviced long life,and the enzyme could not lose easily.Conclusion This method can be used to fix the enzyme on the biosensor.