绒毛中表皮生长因子受体和血管内皮生长因子的表达对早孕绒毛血管生成的影响及与稽留流产的关系

Influence of epidermal growth factor receptor and vascular endothelial growth factor expression on angiogenesis in chorionic villi and its relationship with missed abortion

目的探讨表皮生长因子受体(EGFR)、血管内皮生长因子(VEGF)的表达对早孕绒毛血管生成的影响及与早孕稽留流产的关系。方法应用免疫组织化学方法检测30例稽留流产(稽留流产组)及30例早孕人工流产(早孕人工流产组)绒毛EGFR、VEGF的表达及绒毛的微血管密度(MVD),并分析相关因子的相关性。结果稽留流产组绒毛的MVD(7.03±1.72)比早孕人工流产组(14.74±1.66)显著降低(P<0.01)。稽留流产组绒毛的EGFR表达水平(0.42±0.14)表达比早孕人工流产组(0.49±0.07)低(P<0.05),稽留流产组绒毛的VEGF表达水平(0.47±0.12)表达比早孕人工流产组(0.60±0.12)低(P<0.01)。Pearson相关分析显示在早孕人工流产绒毛及稽留流产绒毛中EGFR与VEGF的表达呈正相关,EGFR的表达与MVD呈正相关,VEGF表达与MVD呈正相关。结论绒毛组织中EGFR和VEGF的表达与绒毛血管生成状况密切相关,EGFR和VEGF低表达参与了稽留流产的绒毛血管的形成障碍。

Objective To investigate the potential roles of epidermal growth factor receptor（EGFR） and vascular endothelial growth factor（VEGF） in villous angiogenesis in the first trimester and its relationship with missed abortion. Methods We detected the expression of EGFR, VEGF and micro vessel density（MVD） by immunohistochemistry in the villi from women with missed abortion group（n=30） and from women with requested induced abortion in the first trimester group（control group）（n=30）. We also analyzed their correlations between the two groups. Results Missed abortion patients were detected significantly lower MVD than control group. Compared with control group, the expressions of EGFR and VEGF were statistically reduced in missed abortion. Correlation analysis showed that the expressions of EGFR and VEGF were positively correlated with MVD in the two groups.Conclusion The expressions of EGFR and VEGF were closely related to villous angiogenesis. EGFR and VEGF were closely involved in abnormal angiogenesis etiology of missed abortion.