不同配比白术茯苓汤对CD大鼠(脾虚型)血清细胞因子水平的影响

Effects of Different Proportions of Atractylodes Macrocephala and Poria Cocos Decoction on Serum Cytokines in Rats With Spleen Deficiency CD

目的探讨白术茯苓汤不同配比对脾虚型克罗恩病(CD)大鼠血清细胞因子的影响。方法选择96只SPF级SD大鼠,分为A组(脾虚型CD造模)、B组(空白组)。A组按照白术茯苓配比分为模型组、A1、A2、A3、A4、A5、A6、A7、A8、A9、A10。检测血清白介素-10(IL-10)、肿瘤坏死因子-α(TNF-α)水平。结果模型组TNF-α高于B组,IL-10低于B组(P<0.05);A5、A6、A7组TNF-α低于模型组(P<0.05);A6组IL-10升高(P<0.05)。结论脾虚CD大鼠血清细胞因子受白术茯苓汤不同配比影响,特别是A6组。

Objective To investigate the effect of different proportions of atractylodes macrocephala and poria cocos decoction on serum cytokines in rats with spleen deficiency Crohn＇s disease（CD）. Methods 96 SPF grade SD rats were selected and randomly divided into A group（spleen deficiency CD model group） and B group（blank group）. The A group was divided into model group, A1, A2, A3, A4, A5, A6, A7, A8, A9 and A10 according to the proportion of atractylodes macrocephala and poria cocos decoction. Serum interleukin-10（IL-10） and tumor necrosis factor alpha（TNF-α） levels were detected. Results TNF-αlevel in model group was higher than B group, IL-10 level was lower than B group（P〈0.05）; TNF-αlevel in A5, A6 and A7 group was lower than that of model group（P〈0.05）; IL-10 level in A6 group increased（P〈0.05）. Conclusion The cytokines in serum of rats with spleen deficiency CD are affected by different proportions of atractylodes macrocephala and poria cocos decoction, especially A6 group.