氨基酸改性壳聚糖抗菌材料制备及其性能研究

Preparation and properties of amino acid modified chitosan antibacterial material

以壳聚糖(CS)为基体,在缩合剂1-(3-二甲氨基丙基)-3-乙基碳二亚胺盐酸盐(EDC·HCl),偶联剂N-羟基-丁二酰亚胺(NHS)的存在下,将L-精氨酸接枝到CS大分子上,制备出一种天然高分子基重金属螯合及抗菌材料,L-精氨酸接枝壳聚糖CS-L-Arginine(CA)。阐述了反应机理,用采用元素分析、拉曼光谱(RAM)、核磁共振谱(13C NMR)以及X射线衍射谱(XRD)分析与表征了产物的结构产物。加入山梨醇作为增塑剂,采用涂膜法制备氨基酸改性壳聚糖膜。实验表明,随着接枝率的提高,改性壳聚糖膜对Ni^(2+)、Cu^(2+)的饱和吸附量的变化趋势先增大再减小,当接枝率为16.85%的改性壳聚糖膜对Ni^(2+)、Cu^(2+)的饱和吸附量达到最优,分别为148.32与198.78mg/g;对大肠杆菌和金黄色葡萄球菌的抑制作用逐渐提高,当接枝率为16.85%时,抑菌效率最佳,可分别达到了92.06%,93.58%。

A new kind of natural-macro-molecular-based heavy metal ion chelating material,L-arginine grafted chitosan（CA）of chelating heavy metal ions,was prepared by grafting L-arginine（L-Arg）onto CS molecules,with chitosan as raw material,1-（3-dimethylaminopropyl）-3-ethylcarbodiimide hydrochloride（EDC.HCl）as condensing agent and N-hydroxysuccinimide（NHS）as coupling reagent.Reaction conditions were optimized.The structure of the product was characterized using elemental analysis,Raman spectroscopy（RAM）,13C NMR spectroscopy（13C NMR）and X-ray diffraction（XRD）analysis.Using sorbitol as plasticizer,the amino acid modified chitosan membrane was prepared by coating method.The results of adsorption experiments showed that with the increase of grafting rate,the change trend of saturated adsorption capacity of modified chitosan film to Ni2＋ and Cu2＋ increased firstly and then decreased.CA（GR=16.85%）membrane had best adsorption capacity of Ni2＋ and Cu2＋,which respectively reached 148.32 mg/g a nd 198.78 mg/g.With the increase of grafting rate,the inhibitory effect on Escherichia coli and Staphylococcus aureus gradually increased.When GR=16.85%,the highest inhibition on Escherichia coli and Staphylococcus aureus respectively reached 92.06% and 93.58%.