HPLC法定量盐碱地红白忍冬和金银花不同器官中5种活性物质

Determination of Five Active Substances in Different Organs of Lonicera japonica var. chinensis( Wats. ) Bak. and Lonicera japonica Thunb. by HPLC Method

[目的]建立同时测定盐碱地种植的红白忍冬和金银花中新绿原酸、绿原酸、咖啡酸、木犀草苷和木犀草素的HPLC方法,并比较不同植物器官中5种成分的含量。[方法]色谱柱为InertsilODS-SP C18柱(250 mm×4.6 mm,5μm);流动相为0.1%甲酸水溶液-乙腈;体积流量1.0 m L/min;检测波长327 nm;柱温30℃。[结果]新绿原酸、绿原酸在1.0~500.0μg/m L,咖啡酸在0.2~100.0μg/m L,木犀草苷、木犀草素在0.1~50.0μg/m L具有良好的线性关系;5种成分的日内和日间精密度RSD值均≤4.2%,且在36 h内稳定,平均回收率为95.60%~97.35%。红白忍冬和金银花药材样品中5种化合物的含量差异较显著,在各器官中金银花5种化合物均比红白忍冬高,但红白忍冬中5种化合物的含量亦有较高水平。[结论]该方法简单便捷,适合于红白忍冬中生物活性化合物的定量测定研究,能为该药材的质量控制制定提供科学、可靠的分析技术。

[Objective] The research aimed to establish HPLC method for simultaneous determination of neochlorogenic acid,chlorogenic acid,cafferic acid,luteoloside and luteolin in L. japonica var. and L. japonica Thunb. cultivated in saline soil,and compare the contents of five components in different plant organs.[Method]The chromatographic column was Inertsil? ODS-SP C18 column( 250 mm × 4. 6 mm,5 μm); the mobile phase was 0. 1% formic acid aqueous solution-acetonitrile; volume flow rate was 1. 0 m L/min; detection wavelength was 327 nm; column temperature was 30 ℃.[Result]Neochlorogenic acid and chlorogenic acid in 1. 0-500. 0 μg/m L,caffeic acid in 0. 2-100. 0 μg/m L,luteoloside and luteolin in 0. 1-50. 0 μg/m L had a good linear relationship; The RSD values of the inter-and intra-precisions were less than4. 2%. The stability was accepted within 36 h. The average recovery rate were 95. 60%-97. 35%. The contents of five compounds in L. japonica Thunb. were higher than those in L. japonica var.,but the contents of five compounds in L. japonica var. was enough.[Conclusion]The method is simple and convenient,and is suitable for the quantitative determination of biologically active compounds in Lonicera japonica var.. It can provide scientific and reliable analytical techniques for the quality control of the medicinal materials.