电感耦合等离子体质谱用于多组分免疫分析研究进展

Development of Multiplex Immunoassay Based on Inductively Coupled Plasma Mass Spectrometry

免疫分析在临床医学检测领域具有重要的地位。本课题组提出了基于电感耦合等离子体质谱(ICPMS)的免疫分析方法,利用元素标记技术结合ICP-MS检测实现多组分免疫分析。随后,研究人员在该领域做了大量研究工作,证明该方法可用于从小分子、蛋白质、核酸到细胞等一系列生物样品的检测。本文综述了基于ICP-MS免疫分析方法的特点,对其发展方向进行了展望,希望为该领域的研究工作提供参考。

Immunoassay is of great importance in the field of clinical detection. Our group proposes the inductively coupled plasma mass spectrometry( ICP-MS) based immunoassay. Multiplex immunoassay can be realized by stable isotope tagging strategy combined with ICP-MS detection. Since then,large amount of researches in this field have been made at home and abroad,and the method has been proved applicable to the analysis of biological small molecules,proteins,nucleic acids and cells. In this article,the history and development of immunoassay,the characters of ICP-MS based immunoassay,single component and multiplex immunoassay,and the magnetic immunoassay that is recently fast developed are reviewed. The prospect of the developing tendency is also anticipated,hoping to provide references for the research in this field.