原子力显微镜-荧光显微镜联用技术在活细胞单分子检测中的应用

Development of Integrated Atomic Force Microscopy and Fluorescence Microscopy for Single-Molecule Analysis in Living Cells

原子力显微镜(Atomic force microscopy,AFM)及荧光显微镜(Fluorescence microscopy,FM)是目前活细胞单分子分析检测中最常用的两种工具。结合两种显微镜的优势,发展高时空分辨、多功能的AFM-FM联用技术成为近年该领域的研究热点。本文简述了AFM单分子力谱和FM单分子荧光成像的原理,总结了AFM-FM联用系统在仪器研制方面的发展概况,并结合本课题组在应用AFM-FM联用技术研究细胞膜上配受体相互作用等方面的工作,介绍了其在活细胞单分子检测中的应用进展。

Atomic force microscope( AFM) and fluorescence microscope( FM) have been emerging as two most commonly used tools for single-molecule study in living cells. Combining the advantages of two microscopes,the development of the integrated AFM-FM technique with high spatiotemporal resolution and multi-function has attracted increasing interest. In this review,the principles of AFM single-molecular force spectroscopy and single-molecule fluorescence imaging were briefly discussed,and the recent advances in the integrated AFM-FM instrumentation were summarized. Subsequently based on our own research in the investigation of ligand-receptors interactions with the integrated AFM-FM technique,its applications in livecell single-molecule imaging and characterization were introduced.