基于Taylor弥散理论的纳米粒子与蛋白质相互作用研究

Study of Interaction of Nanoparticles and Proteins in Taylor Dispersion Analysis

在微流控芯片中将Taylor弥散分析(TDA)与激光诱导荧光检测(LIF)结合,测定了荧光素钠标记狗血清蛋白(FITC-DSA)的水合半径为(6.12±1.21)nm,扩散系数为(4.11±0.78)×10^(-11)m^2/s;然后,初步研究了FITC-DSA与不同粒径金纳米粒子(Au NPs)的相互作用。研究结果表明,不同粒径的Au NPs与蛋白质的作用不同;50 nm的Au NPs与FITC-DSA作用会导致其荧光信号增强。本研究为高通量测定纳米粒子与蛋白质相互作用提供了一种新方法。本方法具有简单快速、耗样量极少等优点,有助于深入了解纳米材料的毒性,推动安全纳米药物的发展。

The diffusion coefficient(( 4.11±0.78) ×10^(-11) m^2/s) and hydrated radius(( 6.12 ± 1.21) nm) of FITC-DSA( fluorescein isothiocyanate conjugate dog albumin) were investigated and measured by combining Taylor dispersion analysis with laser induced fluorescence in microchip. The influence of size of gold nanoparticles( 15,30 and 50 nm) on the interactions between FITC-DSA and gold nanoparticles was studied.The preliminary result demonstrated that the binding of protein and gold nanoparticle displayed a sizedependent relationship. It was interesting that the 50-nm gold nanoparticles could enhance the FITC-DSA fluorescence in microchannel driven with pressure. This method was simple,fast,low sample consumption and high throughput and could be used to study the interaction of nanoparticles and proteins. Systematical study using this method would enable us to have a deep understanding of the toxicity of nanomaterials,and promote the development of safe nanomedicine.