恒河猴大脑中动脉M1段栓塞模型的麻醉新方法

An anesthesia method for the middle cerebral artery M1 segment occlusion model in rhesus monkey

目的为成功构建恒河猴M1段栓塞模型提供简捷、迅速、安全的麻醉方法。方法雄性成年恒河猴25只,年龄7~9岁,体重7~11 kg,由中国人民解放军军事医学科学院实验动物中心提供。以0.1 ml/kg氯胺酮及速眠新的混合液肌肉注射,待动物入睡后气管插管(ID:4.5-5.5#),妥善固定后送入介入中心。入室后连接监护、建立静脉通路并导尿。术中用丙泊酚2~4 mg/(kg·h)持续泵入维持麻醉,呼吸机控制呼吸,并根据动物的生命体征和肢体活动情况调整麻醉深度,必要时追加上述氯胺酮与速眠新的混合液,并根据手术的需要调整心率、血压、体温等。溶栓前后行脑MRI造影,造影前适时停止麻醉药物输注,确保动物在造影室已恢复自主呼吸。术中监测实验动物的心率、体温、血压及血氧饱和度,并于股动脉穿刺及溶栓后抽取动脉血行血气分析。结果所有动物均按预计方案实施完成,术中未发生动物躁动、呼吸抑制、心律失常等严重并发症。实验结束停药后,实验动物很快清醒并送回动物实验中心,进行后续处理。其中18只实验动物存活24 h以上,7只死于溶栓后严重的脑出血和脑梗死。结论以氯胺酮及速眠新的混合液进行麻醉诱导行气管插管,丙泊酚静脉维持全身麻醉可以为此类较复杂的介入及MRI实验的顺利完成提供一种安全、实用的麻醉方法。

Objective To provide a simple, convenient, and safe anesthesia method for the M1 segment of middle cerebral artery occlusion model of rhesus monkeys. Methods Twenty five male rhesusmonkeys, with an average age of 7-9 years and an average body weight of 7-11 kg, were selected for the study. Sumianxin injection combined with ketamine 0. 1 mg/kg was given by intramuscular injection before endotracheal intubation （ID ：4.5-5.5#）. Animals were then transported to a interventional operation room, where the intravenous access was established with the insertion of a urinary catheter. Mechanical ventilation was used during surgery, propofol was continuous injected in a speed of 2-4mg/ （kg. h） , sumianxin-ketamine mixture could be given if necessary to maintain an adequate anesthesia depth. Injection dosage was adjusted according to some vital signs of rhesus like the body movements, physiological parameters, and the demand of surgery. Brain MRI examination was performed before and after thrombolysis. Anesthesia was stopped to let the animals have a spontaneous breath every time before MRI text. Heart rates, temperature, non-invasive blood pressure, SpO2were monitored the whole surgery. Blood samples were taken separately from the radial artery for blood gas analysis after femoral artery puncture and at the time point of thrombolysis, Results All the 25 animals awake soon after surgery, no animal have restlessness, respiratory depression, arrhythmia and other serious complications. Eighteen of themsurvived longer than 24 hours, only 7 died from serious cerebral hemorrhage or large cerebral infarction. Conclusion Endotraeheal intubation general anesthesia is safe and practicable for rhesus monkeys undergoing interventional operation and MRI examination procedures.