球旁细胞光学显微镜下4种染色法结果观察

Four staining methods for observation of juxtaglomerullar cells under a light microscope

目的探讨显示小鼠球旁细胞的适宜染色方法,更好地为教学和科研服务。方法取20只小鼠肾组织做石蜡切片,随机分为4组,分别用HE染色、高碘酸-Schiff氏反应染色、改良结晶紫染色和免疫组织化学染色。结果 HE染色和高碘酸-Schiff氏反应染色不能显示肾球旁细胞;改良结晶紫染色和免疫组织化学均能较好地显示肾球旁细胞(胞质内有特征性的被染色的分泌颗粒)。结论改良结晶紫染色和免疫组织化学染色对球旁细胞有较好的染色效果,但改良的结晶紫染色更为简便、经济、稳定。

Objective To explore an optimal staining method for observation of mouse kidney juxtaglomerullar cells. Methods Kidney tissues were harvested from 20 mice and prepared as paraffin sections. The sections were randomly divided to 4 groups for 4 different stainings. HE, PAS, modification crystal violet and immunohistoehemistry staining, were used for the renal juxtaglomerular cellular secretive granula. Results Neither HE nor PAS methods was able to demonstrate the epithelium-like juxaglomerular cells. Both modification crystal violet method and immunohitochemistry staining methods demonstrated the epithelium-like juxaglomerular ceils containing secretive granula. Conclusion Although both modification crystal violet and immunohistochemistry staining methods are able to show better staining result, the modification crystal violet method is more practical than the immunohistochemistry staining method , because of its convenience, easiness, low-cost and stability.