菖蒲药材的鉴别研究及含量测定

Identification and determination of Acorus tatarinowii by HPLC

目的:建立菖蒲药材中主要成分β-细辛醚、α-细辛醚的HPLC含量测定方法,并对石菖蒲、水菖蒲药材进行质量评价。方法:采用外标法计算菖蒲药材中β-细辛醚、α-细辛醚的含量。色谱条件:Zorbax SB-C18(250mm×4.6mm,5μm),流动相:0.1%甲酸水-甲醇(65:35)等度洗脱,检测波长:257nm,柱温:30°C,流速:1mL·min-1。结果β-细辛醚含量在0.090 45~1.809μg范围内、α-细辛醚含量在0.006 75~0.135μg范围内与峰面积呈良好的线性关系,石菖蒲中β-细辛醚、α-细辛醚平均含量分别为2.18%、0.13%,水菖蒲中β-细辛醚、α-细辛醚平均含量分别0.33%、0.04%。结论:应对市场流通中的菖蒲药材应该进行严格的审查鉴别,避免以次充好、影响临床用药安全及疗效。

A HPLC method for determination of ~asarone and a-asarone was established and the quality of Acorus tatarinowii Schott and Acorus calamus L was evaluated. The quantification content was realized by using the external standard method. The separation was performed on a Zorbax SB-C18 （250 min×4.6 mm, 5μm） column. The mobile phase was isocratic elution of 0.1% formic acid water-methanol. The detection wavelength was set at 237 nm. The column temperature was maintained at 30℃. The flow rate was 1 mL ·min^-1. The mass of β-asarone and α-asarone showed good linearity in the ranges of 0. 090 45- 1. 809μg and 0. 006 75--0. 135μg. The average content of β-asarone and a-asarone in Acorus tatarinowii Schott were 2.18% and 0. 13%, while the average content of β-asarone and α-asarone in Acorus calamus L were 0. 03% and 0. 04%. In market circulation, the medicine ＂Chang Pu＂ should be distinguished and examined strictly to avoid the circumstance of adulterate and the damage to safety of drug use.