汞离子胶体金免疫层析试纸条的研制

An Immunochromatographic Assay for Rapid and Direct Detection of Hg^(2+) in Water Samples

基于特异性识别汞离子的单克隆抗体,以胶体金标记抗体作为检测探针,样品中的汞离子和检测线上的包被抗原竞争与免疫探针结合,建立了一种快速测定水样中汞离子的胶体金免疫层析试纸条方法.在最优实验条件下,试纸条对汞离子的检出限为100μg/L,与其他金属离子没有交叉反应,样品的加标回收实验结果与酶联免疫吸附分析方法(ELISA)有很好的相关性.整个分析过程在10min内可以完成,表明该方法可以快速直接检测汞离子,不需要复杂的样品预处理过程.

In this paper,based on the monoclonal antibody（mAb）which can specifically recognize individual Hg^2＋,a competitive immunochromatographic assay（ICA）using a colloidal gold-mAb probe for rapid and direct detection of Hg^2＋in water samples is described.The mercury cations（Hg^2＋）in the standard/sample solution compete with the coating antigen immobilized on the test line for limited mAb on the immunoprobe.Under optimal conditions,the assay for Hg^2＋ can be completed within 10min,with a visual limit of detection（LOD）for Hg^2＋ of 100μg/L and no cross-reactivity with other metal ions.In a spiked recovery experiment,real water samples spiked with different concentrations of Hg^2＋ were measured by the proposed ICA,and the results were highly consistent with those obtained from enzyme-linked immunosorbent assay（ELISA）.It is hence demonstrated that the proposed ICA is an applicable method for rapid and direct detection of Hg^2＋ on-site without complexing pretreatment steps.