牛蒡子提取物中咖啡酰奎宁酸类化合物的分离与结构确证

Isolation and Identification of Caffeoylquinic Acid Compounds in the Extractive of Fructus arctii

采用乙酸乙酯萃取法从牛蒡子提取物中除去脂溶性成分,再取水相干燥物用制备型HPLC分离,共得到6个化合物,并通过理化性质与光谱方法进行了结构确证,分别为3,4-二羟基苯丙烯酸-4-氧-β-D-葡萄糖苷、3-O-咖啡酰奎宁酸、3,4-二羟基苯丙烯酸、3,5-二咖啡酰奎宁酸、1,5-二咖啡酰奎宁酸和3,4-二咖啡酰奎宁酸.

The fat soluble components in the extractive of Fructus arctii were removed by ethyl acetate,the aqueous phase drying was isolated by preparative HPLC,and six compounds were isolated from the extractive of F.arctii and their structures were identified by physicochemical property analysis,specially by spectral analysis.Their structures were identified as 3,4-dihydroxy cinnamic acid-4-O-β-D-glucoside,3-Ocaffeoylquinic acid,3,4-dihydroxy cinnamic acid,3,5-dicaffeoylquinic acid,1,5-dicaffeoylquinic acid and3,4-dicaffeoylquinic acid.Isolation of caffeoylquinic acid compounds from the extractive of F.arctii has not been reported in related literature,and the results of this study may provide support for the research of F.arctii and F.arctii-based preparations.