新疆和河南红花中羟基红花黄色素A和山奈素含量分析

Analysis of Hydroxysafflor Yellow A and Kaempferol in Carthamus Tinctorrius L. by Xinjiang and Henan

目的:高效液相色谱法(HPLC)方法测定新疆和河南红花不同产地、不同品种羟基红花黄色素A和山奈素含量,为评价新疆和河南不同产地和不同品种红花质量提供参考依据。方法:采用高效液相色谱法对新疆44批、河南4批,共48批红花样品进行羟基红花黄色素A测定,对新疆30批样品进行山奈素含量测定。结果:48批不同产地、不同品种红花药材羟基红花黄色素A含量为1.037~2.622,30批新疆样品中山奈素含量为0.288~0.739,昌吉州奇台县云南大头红花羟基红花黄色素A含量平均最高,达2.518%,察布查尔锡伯自治县红花山奈素平均含量最高达0.5876%。结论:不同产地、不同品种红花药材羟基红花黄色素A含量整体趋势为新疆>河南,其中新疆三大产区和乌鲁木齐市红花含量趋势为昌吉州地区>塔城地区>乌鲁木齐市>伊犁地区,山奈素含量整体趋势为伊犁地区>塔城地区>昌吉地区,综合分析新疆昌吉地区红花中羟基红花黄色素A和山奈素含量均较好。

Objective： To measure the contents of hydroxysafflor yellow A and kaempferol of Carthamus tinctorrius L. by HPLC from Xinjiang and Henan of different varieties and producing area and provide comprehensive evaluation of their quality. Methods： HPLC method was used to compare 44 batch from Xinjiang, 4 batch from Henan to measure the contents of hydroxysafflor yellow A, 30 batch from Xinjiang to measure the contents of kaempferol. Results ： The contents of hydroxysafflor yellow A from Xinjiang and Henan of different varieties and producing area 48 batch 1.037-2.622, The contents of kaempferol 31 batch 0.288-0.739, The contents of hydroxysafflor yellow A from Changji Prefecture Qitai County Yunnan Datou average content the highest, reach 2.518%, Conculsion ： The contents of kaempferol from Chahuchaer County average content the highest, reach 0.5876%. The contents of hydroxysafflor yellow A from Xinjiang and Henan of different varieties and producing area overall trend： Xinjiang〉Henan, three major producing areas trend Changji〉Tacheng〉Urumqi〉Yili district, The contents of kaempferol overall trend Yili〉Tacheng〉Changji district, Analysis by synthesis the contents of hydroxysafflor yellow A and kaempferol of Carthamus tinctorrius L. from Xinjiang Changji Prefecture is all good.