爵床药材质量标准研究

Study on Quality Standard of Herba Justiciae

目的:建立爵床药材的质量标准。方法:参考2015年版《中国药典》方法,对爵床药材的质量控制方法进行研究,包括水分检查、总灰分和酸不溶性灰分检查、薄层鉴别、显微鉴别和其中有效成分爵床脂定B的含量测定。结果:爵床水分含量为9.04%~10.41%,平均值为9.81%;爵床总灰分含量为15.31%~19.86%,平均值为18.32%;酸不溶性含量为0.15%~0.98%,平均值为0.44%;爵床药材的TLC图谱斑点清晰,分离度良好;显微鉴别特征明显;爵床脂定B在2.048~16.384μg·mL^(-1)浓度范围内与峰面积呈良好的线性关系,r=0.999 5;平均回收率为97.23%(RSD=1.47%,n=6)。结论:该方法操作简单、准确可靠,可用于爵床药材的质量控制。

Objective：To establish the quality standard of Herba Justiciae. Methods：The researches included water inspection, total ash and acid insoluble ash inspection, thin layer identification, microscopic identification and determination of Justicidin B. Results：The moisture was 9.04%- 10.41%,the average was 9.81%; the total ash was 15.31% -19.86%,the average was 18. 32% ; the acid insoluble ash was 0.15% -0.98% ,the average was 0.44%. The TLC plot of Herba Justiciae was clear and the resolution was good. The microscopic identification was obvious. The linear ranges of justicidin B is 2. 048- 16. 384 gg/mL （ r = 0. 999 5）. The average recoveries of the component is 97.23 % （RSD=1.47 %）. Conclusion： The method can he used for the quality control of Herba Justiciae.