响应面法优化茯苓总三萜超声波提取工艺

Optimization of Ultrasonic Extraction Process of Total Triterpenoids from Poria Cocos (Schw.) Wolf by Response Surface Methodology

目的 :优选茯苓总三萜的超声波提取工艺。方法 :在单因素考察的基础上,以茯苓中总三萜的得率为评价指标,采用响应面分析法(选取乙酸乙酯为溶剂,料液比、提取温度、提取时间)确定最佳的超声波辅助提取工艺。结果 :最佳工艺为30倍体积的乙酸乙酯,超声时间32 min,超声温度35℃,茯苓总三萜的得率为0.685%。验证试验结果为0.683%,与预测值接近。结论 :Box-Behnken-设计的响应面优化茯苓总三萜的提取工艺方法简单,合理,可预测性优。

Objective ：To optimize the ultrasonic-assist extraction process of total triterpenoids from Poria cocos. Methods ：Response surface methodology was applied to optimize the best extraction conditions with the yield of total triterpenoids as index,on the basic of single factor tests,including liquid-solid ratio,extracting temperature and time. Results ：The optimal conditions as follows ：solidliquid ratio at 30∶1,the time for 32 min,extracting temperature at 35 ℃. The extraction rate of total triterpenoids from Poria cocos was 0.685%. The result of verification test was 0.683%,which was closed to the predicted value. Conclusion ：The process is simple,reasonable and predictable,which can be used as the extraction process of triterpenes in P. cocos.