XCT-790对沉默Dkk1腺病毒载体转染MG63细胞活性和OPN、Lrp5、BMP2影响研究

Effect of XCT-790 on the Activity and OPN,Lrp5,BMP2 of MG63 Cells Infected with Silence Dkk1 Adenovirus Vector

目的 :研究XCT-790对沉默Dickkopf-1(Dkk1)腺病毒转染MG-63细胞活性和OPN、Lrp5、BMP2的影响。方法 :构建沉默Dkk1腺病毒载体,实验分为空白对照组、沉默Dkk1组、XCT-790处理空载腺病毒组、XCT-790处理沉默Dkk1组,根据组别不同分别用上述重组腺病毒转染MG63细胞,观察细胞增殖及OPN、Lrp5、BMP2表达情况。结果 :(1)与空白对照组比较,沉默Dkk1组和XCT-790处理空载腺病毒组MG63细胞活性增强,OPN、Lrp5、BMP2的表达量升高((P<0.05);(2)与Dkk1沉默组比较,XCT-790处理Dkk1沉默组的MG63细胞活性减弱,OPN、Lrp5、BMP2的表达量降低(P<0.05)。结论 :XCT-790可以降低因DKK1沉默而增强的MG63细胞活性,可以降低因DKK1沉默而增高的OPN、Lrp5、BMP2的表达量。

Objective ：To investigate the effect of XCT-790 on the activity and OPN,Lrp5,BMP2 of MG63 cells infected with silence Dickkopf-1（Dkk1）adenovirus vector. Methods ：Constructed recombinant adenovirus vector of silencing about Dkk1,the experiment was divided into control group,silence Dkk1 group,XCT-790 dispose no-load adenovirus group,XCT-790 dispose silence Dkk1 group,use these recombinant adenovirus to infect MG63 cells,then observe the cell proliferation and expression of OPN,Lrp5,BMP2. Results ：（1）Compared with the control group,the activity and the expression of BMP2,Lrp5,OPN of MG63 cells in silence Dkk1 group and XCT-790 dispose no-load adenovirus group was increased（P0.05）;（2）Compared with the silence Dkk1 group,the MG63 cell activity and expression of BMP2,Lrp5,OPN of XCT-790 dispose silence Dkk1 group both reduced（P0.05）. Conclusion ：XCT-790 can reduce effect of the higher MG63 cell activity and expression of BMP2,Lrp5,OPN that due to the silence Dkk1.