三七叶的化学成分及其抗氧化活性研究

Chemical constituents from leaves of Panax notoginseng and anti-oxidant activity

目的研究三七Panax notoginseng叶乙醇提取物的化学成分及抗氧化活性(ABTS和DPPH)。方法利用硅藻土、硅胶、MCI柱色谱、凝胶柱色谱、中压制备液相色谱和制备液相等色谱技术进行分离纯化,并应用质谱、核磁共振等波谱技术鉴定化合物结构。初步考察了这些化合物的抗氧化能力。结果从三七叶95%乙醇提取物的氯仿、丙酮-乙醇(4∶1)和丙酮-乙醇(1∶1)3个洗脱部位中分离鉴定了15个化合物,分别为falcarinol(1)、γ-tocopherol(2)、(5S)-3α-acetyl-2,3,5-trimethyl-7α-hydroxy-5-(4,8,12-trimethyltridecanyl)-1,3α,5,6,7,7α-hexahydro-4-oxainden-1-one(3)、picrionoside B(4)、linarionoside A(5)、viburnolide A(6)、槲皮素(7)、lilyn(8)、茵芋苷(9)、阿彼斯基姆素(10)、daphnin methylether(11)、20(S)-人参皂苷Rh2(12)、人参皂苷F2(13)、20(R)-人参皂苷Rh2(14)、异人参皂苷Rh3(15)。结论化合物3、6、9~11为首次从人参属植物中分离得到,化合物2、4为首次从该植物中分离得到。化合物2、4、7、8具有较强的抗氧化活性。

Objective To study the chemical constituents from the leaves of Panax notoginseng and their antioxidant activity（ABTS and DPPH）.Methods The constituents were isolated and purified by celite,silica gel,MCI and Sephadex LH-20,reversed-phase MPLC chromatographic methods,then purified by preparative HPLC.Their structures were identified on the basis of mass spectrum and NMR spectrum.Anti-oxidant activity of these compounds was initially investigated.Results Fifteen compounds were isolated from the chloroform,acetone-ethanol（4：1） and acetone-ethanol（1：1） fractions of 95% ethanol extract of the leaves of P.notoginseng,and their structures were elucidated as follows：falcarinol（1）,γ-tocopherol（2）,（2 S,4αS,7αR）-7α-acetyl-3,4,4α,7α-tetrahydro-4α-hydroxy-2,6,7-trimethyl-2-（4,8,12-trimethyltridecyl） cyclopenta [β] pyran-5（2 H）-one（3）,picrionoside B（4）,linarionoside A（5）,viburnolide A（6）,quercetin（7）,lilyn（8）,skimmin（9）,apiosylskimmin（10）,daphnin methylether（11）,20（S）-ginsenoside Rh2（12）,ginsenoside F2（13）,20（R）-ginsenoside Rh2（14）,and isoginsenoside Rh3（15）.Conclusion Compounds 3,6,and 9—11 are isolated from the plant of genus Panax for the first time,compounds 2 and 4 are isolated from this plant for the first time.Compounds 2,4,7,and 8 show certain anti-oxidant activity.