细叶杜香中酚类成分及其抗氧化活性研究

Phenolic constituents of Ledum palustre and their anti-oxidant activity

目的研究细叶杜香Ledum palustre全草的化学成分。方法采用硅胶柱色谱和高效液相色谱等分离方法,对细叶杜香醋酸乙酯萃取物的化学成分进行分离并经波谱数据分析鉴定化合物的结构,并通过清除DPPH实验探讨其抗氧化活性。结果从细叶杜香全草无水乙醇提取液醋酸乙酯萃取物中分离得到14个酚类化合物,分别鉴定为白藜芦醇(1)、桑辛素M(2)、儿茶素(3)、表儿茶素(4)、莨菪亭(5)、七叶内酯(6)、秦皮素(7)、5-羟基-2-甲氧基苯甲酸(8)、秦皮啶(9)、2α,3α-epoxy-5,7,3′,4′-tetrahydroxyflavan-(4β-8)-epicatechin(10)、2α,3α-epoxy-5,7,3′,4′-tetrahydroxyflavan-(4β-8-catechin)(11)、臭矢菜素A(12)、臭矢菜素C(13)、异秦皮苷(14)。结论化合物1、2、8~13为首次从杜香属植物中分离得到;化合物3、4、6、7、10、11具有显著的抗氧化活性。

Objective To study the chemical constituents of Ledum palustre.Methods Compounds were isolated from n-hexane fraction in the ethanol extract from L.palustre by silica gel column chromatography and HPLC.The structures of the chemical constituents were identified by analysis on their spectral data.Results Fourteen compounds were isolated from Et OAc fraction in the ethanol extract from L.palustre and identified as resveratorol（1）,moracin M（2）,catechin（3）,epicatechin（4）,scopoletin（5）,esculetin（6）,fraxetin（7）,5-hydroxy-2 methoxybenzoic acid（8）,fraxidin（9）,2α,3α-epoxy-5,7,3′,4′-tetrahydroxyflavan-（4β-8）-epicatechin（10）,2α,3α-epoxy-5,7,3′,4′-tetrahydroxyflavan-（4β-8-catechin）（11）,cleomiscosin A（12）,cleomiscosin C（13）,and isofraxoside（14）.Conclusion Compounds 1,2,and 8—13 are obtained from the plants of Ledum L.for the first time.Compounds 3,4,6,7,10,and 11 had significant anti-oxidative activities.