脱脂肉桂粉提取肉桂多酚的研究

Study on extraction of cinnamon polyphenols from defatted cinnamom bark powder

目的研究用脱脂肉桂粉为原料提取肉桂多酚的工艺条件。方法肉桂经超临界CO_2萃取得到脱脂肉桂粉,脱脂肉桂粉用乙醇回流提取后,经滤过、浓缩、真空干燥即得肉桂多酚提取物。考察提取时间、乙醇体积分数、料液比、提取温度对肉桂多酚提出率的影响。结果脱脂肉桂粉中肉桂多酚的最佳提取条件为时间2 h,乙醇体积分数55%,料液比1∶8,温度60℃。在此提取条件下,其多酚提出率为106.4 mg/g;肉桂多酚提取物(干物)的产率为20.7%,其多酚质量分数为51.4%,其香豆素质量分数为104μg/g。结论采用超临界CO_2萃取技术对肉桂粉进行脱脂处理,可以大幅度提高肉桂多酚的提出率,大大降低肉桂多酚提取物中香豆素的量。

Objective To research the extraction conditions of cinnamon polyphenols with defatted cinnamon bark powder.MethodsCinnamon bark powder was extracted by supercritical carbon dioxide extraction to de-fat firstly and then the effects of extraction time,ethanol concentration,ratio of feed to liquid,and extraction temperature on the extraction ratio of polyphenols were investigated in detail.Results The result shows that the optimum extraction conditions of cinnamon polyphenols from the defatted cinnamon bark powder are：extraction time 2 h,ethanol concentration 55%,feed liquid ratio 1∶8,and temperature 60 ℃.Under the above conditions,the extracted ratio of the polyphenols from the defatted cinnamon bark powder went up to 106.4 mg/g and the yield of cinnamon bark extract went up to 20.7%.The content of polyphenols in the dry extract was 51.4%,which was much higher than that from the original cinnamon powder while the content of coumarin of the extract was 104 μg/g.Conclusion The defatting of cinnamon powder by supercritical carbon dioxide extraction in advance can greatly increase the yield of cinnamon polyphenols and reduce the content of coumarin in the cinnamon bark extract.