水栀子中酚性成分研究

Phenolic constituents from fruit of Gardenia jasminosides var. radicans

目的研究水栀子Gardenia jasminosides var.radicans的酚性成分。方法采用硅胶、Toyopearl HW-40、Sephadex LH-20、MCI gel CHP-20、ODS柱色谱法及制备液相进行分离纯化,根据理化性质和波谱数据鉴定化合物结构。结果从水栀子醋酸乙酯部位分离得到17个酚性化合物,分别鉴定为3,3′,5-trimethoxy-9,9′-epoxylignane-4,4′-diol(1)、3,4-二香草酰基四氢呋喃(2)、marphenol C(3)、芥子醛(4)、对羟基肉桂酸(5)、异阿魏酸(6)、咖啡酸(7)、异秦皮啶(8)、异莨菪内酯(9)、6-甲氧基-7-羟基香豆素(10)、6,7-二羟基香豆素(11)、丁香酸(12)、3-羟基-4-甲氧基苯甲酸(13)、3,4-二羟基苯甲酸(14)、对羟基苯甲酸(15)、没食子酸-3-甲基醚(16)、没食子酸(17)。结论以上化合物均为首次从水栀子中分离得到。

Objective To investigate the phenolic constituents in the fruit of Gardenia jasminosides var. radicans. Methods The phenolic constituents were isolated and identified by chromatography on silica gel, Toyopearl HW-40, Sephadex LH-20, MCI gel CHP-20, ODS, and RP-HPLC. Their structures were elucidated on the basis of physicochemical properties and spectral analyses. Results Seventeen compounds were isolated from 50% aqueous acetone extract from the fruit of Gardenia jasminosides var. radicans and identified as 3,3′,5-trimethoxy-9,9′-epoxylignane-4,4′-diol（1）, 3,4-divanillyltetrahydrofuran（2）, marphenol C（3）, sinapaldehyde（4）, hydroxycinnamic acid（5）, isoferulic acid（6）, caffeic acid（7）, isofraxidin（8）, isoscopoletin（9）, 6-methoxy-7-hydroxycoumarin（10）, 6,7-dihydroxycoumarin（11）, syringic acid（12）, 3-hydroxy-4-methoxybenzoic acid（13）, 3,4-dihydroxybenzoic acid（14）, p-hydroxybenzoate（15）, 4,5-dihydroxy-3-methoxy-benzoic acid（16）, and gallic acid（17）. Conclusion All 17 compounds are isolated from this plant for the first time.