糙叶五加叶中总黄酮的纯化工艺研究

Purification of total flavonoids from leaves of Acanthopanax henryi

目的研究优化糙叶五加叶总黄酮的大孔吸附树脂纯化工艺。方法以处理后的总黄酮的质量分数和总黄酮得率为考察指标,采用单因素实验结合响应曲面设计法进行纯化工艺优化研究。结果 D101型大孔吸附树脂对糙叶五加叶总黄酮有较好的吸附和洗脱效果,其最佳的纯化工艺条件为色谱柱的径高比为1∶10,25.0 g D101大孔树脂的上样量为750 mg,溶剂体系为50%乙醇,洗脱体积流量为5 m L/min,洗脱体积为130 m L。在此最佳优化条件下,糙叶五加叶总黄酮的理论质量分数为75.69%,实际质量分数达到75.87%;总黄酮得率为30.13%。结论 D101型大孔吸附树脂宜于纯化糙叶五加叶中的总黄酮,且响应面法能有效地优化其纯化工艺。

Objective To optimize the purification technology of total flavonoids from the leaves of Acanthopanax henryi by macroporous resin. Methods Using the purity and yield of total flavonoids as indexes, the single factor experiment combined with response surface methodology（RSM） was used to optimize the purification technology. Results It showed that D101 macroporous resin had good adsorption and desorption effects. The optimal purification conditions were as follows： diameter height ratio was 1∶10, the loading amount was 750 mg each 25 g D101 macroporous resin, the flow rate was 5 mL/min, and eluted by 130 mL 50% ethanol. Under the proposed conditions, the experimental purity of total flavonoids reached 75.87%, which was well matched with the predictive purity of 75.69%. And the yield of total flavone was 30.13%. Conclusion The results proved that D101 macroporous resin can purify the total flavonoids from the leaves of A. henryi and RSM could optimize the purification technology effectively.