香青兰总黄酮对D-半乳糖/亚硝酸钠诱导痴呆小鼠学习记忆的影响

Effects of total flavonoids of Dracocephalummoldavica L. on learning and memory disorders of mice with dementia induced by D-galactose/sodium nitrite

目的:观察香青兰总黄酮对D-半乳糖/亚硝酸钠诱导的痴呆小鼠学习记忆的影响。方法:皮下连续注射D-半乳糖(120 mg·kg-1)/亚硝酸钠(90 mg·kg-1)60 d建立痴呆小鼠模型,观察受试药(25,50和100 mg·kg-1)对痴呆小鼠学习记忆的影响及对脑组织中SOD,MDA,GSH-PX,IL-1β,TNF-α和i NOS含量的影响。结果:与模型组相比,香青兰总黄酮对痴呆小鼠学习记忆有明显的改善作用;其中剂量组可显著降低MDA和IL-1β水平;高剂量组可明显提高SOD活性,下调MDA,IL-1β和TNF-α表达。结论:香青兰总黄酮可显著改善痴呆小鼠的学习、记忆能力,其机制可能与其降低脑组织中氧化水平、下调炎症介质的表达有关。

Objective： To observe the effects of total flavonoids of Dracocephalummoldavica L. on D-galactose/sodium nitrite-induced learning and memory disorders in mice. Methods： Sodium nitrite and D-galactose were subcutaneously injected for 60 days to induce dementia model in mice. The effect of total flavonoids of Dracocephalummoldavica L（25,50 and 100 mg·kg-1） on the learning and memory disorders in mice were evaluated by Morris water maze,and the levels of superoxide dismutase（SOD）,malondialdehyde（MDA）,glutathione peroxidase（GSH-Px）,interleukin-1β（IL-1β）,tumor necrosis factor-α（TNF-α） and nitric oxide synthase（i NOS） in the cortex were measured. Results： Compared with the model group,treatment with total flavonoids from Dracocephalummoldavica L. significantly improved the learning and memory deficits induced by D-galactose/sodium nitrite; the dose of 50 mg·kg-1 decreased the expression of MDA and IL-1β; the dose of 100 mg·kg-1 could obviously down-regulate the activity of SOD,and up-regulated the expressions of MDA,IL-1β and TNF-α. Conclusion： Total flavonoids from Dracocephalummoldavica L. can improve the ability of learning and memory in dementia mice and the mechanism is probably related to resisting oxidation damage and inflammatory reaction.