口腔黏膜黑色素瘤人源性移植瘤模型的建立及鉴定

Establishment and validation of patient-derived xenograft model of oral mucosal melanoma

目的:建立和鉴定口腔黏膜黑色素瘤(oral mucosal melanoma,OMM)人源性移植瘤(patient-derived xenograft,PDX)模型。方法:将4例原发性口腔黏膜黑色素瘤组织移植于免疫缺陷裸鼠皮下,观察并记录裸鼠成瘤情况,待移植瘤稳定生长后进行传代;利用H-E染色观察原发瘤和移植瘤的组织病理学特征,应用免疫组织化学染色检测黑色素瘤标志物HMB-45、Melan-A、S-100及Ki-67在两者组织的表达水平。结果:4例口腔黏膜黑色素瘤组织皮下移植成功并序列传代;H-E和免疫组织化学染色显示,移植瘤在组织病理形态和4个标志物表达情况上与原发瘤保持一致。结论:本研究成功建立4例口腔黏膜黑色素瘤人源性移植瘤模型,传代移植瘤保留了原发瘤的组织病理学和分子特征,可为口腔黏膜黑色素瘤研究提供理想的临床前动物模型。

PURPOSE： To establish and validate patient-derived xenograft （PDX） model of oral mueosal melanoma （OMM）. METHODS： Four primary OMM specimens were implanted into immune-deficient nude mice subcutaneously, the mice were evaluated for tumor growth, stable xenograft tumor models and were passaged. Histopathologieal characteristics were observed by H-E staining. OMM-associated clinical markers （HMB-45, Melan-A, S-100 and Ki-67） were detected by immunohistochemistrical staining. RESULTS： OMM samples from 4 patients were implanted subcutaneously and passaged for expansion successfully. Histopathologically and immunohistochemistrieally, PDX models showed similar morphology and HMB-45, Melan-A, S-100 and Ki-67 protein expression to the primary tumor. CONCLUSIONS： Four xenograft models were successfully established, in which the histopathologieal and molecular features of the primary tumors are preserved through passaging, which provid promising preelinical models for translational research of OMM.