促进天麻等兰科药用植物种子萌发的真菌酯酶同工酶研究

Esterase isoenzyme of fungi promoting seed germination of Gastrodia elata etc medcinal plants of Orchidaceae

用聚丙烯酰胺凝胶电泳枝术,对促进天麻等兰科药用植物种子萌发的6种真菌进行了酯酶同工酶分析,获得了各自稳定的同工酶谱。其中5种真菌的酯酶与已知的紫萁小菇相比,酶带数及迁移率均有差异,故其分类地位有待进一步鉴定。同时对酶谱变化与真菌生物学特性的相关性进行了阐述。

Esterase isoenzyme of six strains of fungi whichcou1d promote the seed germination of Gastrodia elataetc medicinal plants of Orchidaceae were determindedby the technique of gel electrophoresis. By compari-son of the enzyme patterns of five strains with thoseof Mycena osmundicola, difference was observed inband numbers and mobility of these fungi. So theclass of species of the five fungi needs to be furtherstudied. The correlations of the changes of enzymepetterns with the characteristics and phylogeny offungi were analyzed.