利用碱-酶耦合法预处理竹粉制备羧甲基纤维素钠

Preparation of sodium carboxymethyl cellulose by pretreatment of bamboo powder with alkali enzyme coupling method

以竹粉为原料,经碱-纤维素酶耦合法预处理,提取纤维素,制备羧甲基纤维素钠。碱液-纤维素酶耦合处理的工艺为:竹粉4 g,5%Na OH 40 m L,碱解温度为50℃,时间1 h,减压过滤后加入400 U/m L酶液30 m L,酶的最适p H为5.5,酶解温度为49℃,时间30 min。经该工艺处理,竹粉中的α-纤维素含量达84.1%,以此为原料制备的CMC 2%浓度水溶液粘度值达162 m Pa·s,取代度为0.95,红外光谱检测显示产物具有羧甲基特征峰,扫描电镜检测显示产物具有盐的特征。本研究在原料预处理上采用生物-化学耦合方法,对环境污染少,成本相对较低,促进了绿色经济的发展,也为制备羧甲基纤维素钠提供了新的途径。

The bamboo powder was pretreated to increase the product＇ s cellulose content, then the sodium carboxymethyl cellulose（CMC） was prepared by the pretreated material. The process of alkali- cellulase coupling method were as follows： bamboo powder 4 g, 5% NaOH 40 mL, alkali hydrolysis at 50 ℃ for 1 h. After vacuum filtration, adjusted pHS.5, adding 400 U/mL cellulase solution 30 mL, then the enzymatic reaction kept at 49℃ for 30 min. After the process, the content t^-cellulose of the treated bamboo powder was 84.1% , and the viscosity of the 2% CMC solution made from this material attained 162 mPa＂ s, the degree of substitutin （DS）was 0.95.The infrared spectroscopy showed that the product had characteristic peak of carboxymethyl group, scanning electron microscopy showed that the product had salt characteristics.There is less environmental pollution and relatively low cost by adopting the bio-ehemical coupling method in the pretreatment of raw materials, and it would promote the development of green economy and provide a new way for the preparation of CMC.