高温蒸煮结合酶解改性枣渣膳食纤维

Technology optimization on steam-cooking combing with enzymatic hydrolysis modification of dietary fiber from jujube residues

为探讨高温蒸煮结合纤维素酶酶解改性枣渣水不溶性膳食纤维的工艺。以枣渣为原料,采用高温蒸煮、纤维素酶酶解改性枣渣水不溶性膳食纤维,以水溶性膳食纤维得率为指标,在单因素实验基础上,采用Box-Behnken中心组合设计,通过响应面法优化高温蒸煮结合酶解改性工艺条件。结果表明:枣渣水不溶性膳食纤维经120℃高温蒸煮60 min,纤维素酶改性枣渣水不溶性膳食纤维最佳工艺条件为酶浓度0.55%、p H4.6、料液比1∶27 g/m L、酶解温度43℃,酶解时间2.5 h,在此条件下水溶性膳食纤维得率为20.03%±0.58%,与模型预测值20.37%较为一致。响应面回归方程与实验结果拟合性好,说明此模型合理可靠,可为枣渣水不溶性膳食纤维改性的工业化应用提供一定参考。

The study aimed to discuss the technology of steam-cooking combining with cellulase hydrolysis on modification of insoluble dietary fiber.Extraction of IDF from jujube residues, using SDF yield as the index, the processing technologies of steam -cooking and eellulase modification were studied. Based on the single factor experiment, the technology optimization was by Box-Behnken central composite design and response surface methodology. The resuhs showed that the steam- cooking technology of IDF from jujube residues was as follows：steam-cooking tempdrature 120℃ and treatment time 60 rain, and the optimal technology for hydrolyzed of IDF by cellulase was determined as follows：enzyme concentration 0.55%, pH4.6,1iquid- solid ratio 1：27 g/mL, enzymatic hydrolysis temperature 43℃ and treatment time 2.5 h.Under these technological conditions, the SDF yield by combining steam-cooking and enzymatic hydrolysis was up to 20.03 % ± 0.58%, which was very close to the predicted value of 20.37% .The regression equation of response surface was in good agreement with the experimental results, indicating that the model was reliable and will provide a reference for the industrial modification of IDF from jujube residues.