榄香烯对神经母细胞瘤增殖的抑制作用

Mechanism of Inhibition of Neuroblastoma Cell Proliferation by Elemene

目的观察榄香烯对神经母细胞瘤增殖的抑制作用,并探讨其作用机制。方法培养SK-N-SH神经母细胞瘤细胞株。采用MTT法测定榄香烯对SK-N-SH细胞的抑制率,应用实时qPCR和Western blotting法测定榄香烯对SK-N-SH细胞中神经元限制性沉默因子(REST)的调控作用,并进一步用实时qPCR检测SK-N-SH细胞中细胞周期相关基因CCND1和CCNE1的表达。结果榄香烯对SK-N-SH细胞的增殖有明显的抑制作用(P=0.001 16),并呈时间和浓度依赖性;榄香烯下调REST mRNA(P=0.000 38)和蛋白表达(P=0.003 39),并且抑制细胞周期相关基因CCND1 mRNA表达(P=0.001 91)和CCNE1 mRNA表达(P=0.000 15)。结论榄香烯对神经母细胞瘤的增殖有抑制作用,并且抑制其REST和细胞周期相关基因CCND1和CCNE1的表达。

Objective To investigate the effect of elemene and the mechanism of this effect on the proliferation of neuroblastoma. Methods We treated cultured SK-N-SH cells with elemene and used the MTT assay to determine the inhibition of cell proliferation. We used RT-qPCR and Western blotting to measure the effect of elemene on mRNA and protein expression,respectively,of repressor element-1 silencing transcription factor （ REST ） in SK-N-SH cells and evaluated the mRNA expression of CCND1 and CCNE1 in SK-N-SH cells by using RT-qPCR. Results Elemene significantly inhibited the proliferation of SK-N-SH cells in a dose- and time-dependent manner （ P = 0.001 16）. Moreover,the mRNA （ P = 0.000 38） and protein （ P = 0.003 39） expression of REST were markedly suppressed by elemene. Furthermore, elemene significantly reduced the mRNA expression of C CND 1 （ P = 0.001 91 ） and CCNE1 （ P = 0.000 15 ） , which are related to the cell cycle. Conclusion Elemene significantly suppressed the proliferation of neuroblastoma cells through the reduction of REST, CCND1, and CCNE1. Keywords