DZNep通过下调EZH2蛋白表达抑制人前列腺癌PC3细胞增殖

Inhibiting effect of DZNep on proliferation of human prostatic cancer PC3 cells by down-regulating EZH2 expression

目的研究EZH2蛋白抑制剂DZNep对前列腺癌PC3细胞增殖的影响及其用于人前列腺癌治疗的潜在价值。方法 MTT分析DZNep处理、siRNA敲低EZH2和EZH2过表达对PC3细胞增殖的影响,Western blot检测EZH2和Bax/Bcl-2的表达。结果 DZNep抑制PC-3细胞增殖、诱导细胞凋亡,上调Bax基因表达并下调Bcl-2基因表达。敲低EZH2抑制PC3细胞增殖,过表达EZH2则拮抗DZNep对PC3细胞的增殖抑制。结论 DZNep通过下调EZH2表达、诱导细胞凋亡发生从而抑制PC3细胞增殖。DZNep具有抗人前列腺癌治疗的潜在应用前景。

Objective To study the effects of S-adenosyl-homocysteine hydrolase inhibitor DZNep （3-Dea- zaneplanocin A） on human prostatic cancer PC3 cells growth and further explore its potential value in anti-human prostatic cancer treatment. Methods MTT method was used to analyze the effects of EZH2 gene silencing, EZH2 over-expression and DZNep treatment on cell proliferation. Gene expression of EZH2, Bax and Bcl-2 in PC3 cells was detected with western blot. Results DZNep could significantly inhibit PC3 cell growth and induce apoptosis which was identified with Bax expression up-regulation and Bcl-2 expression down-regulation. EZH2 knock-down in-hibited PC3 cells growth, and over-expression of EZH2 partially counteract the inhibitory effects of DZNep on PC3 ceils growth. Conclusions DZNep can inhibit PC3 cells growth by targeting EZH2 inhibition which leads to endogenous apoptosis. DZNep has the potential value in the treatment of human prostatic cancer.