摘要
目的研究可溶性环氧化物水解酶(EPHX2)基因rs 751141单核苷酸多态性(SNP)与冠心病的相关性。方法选择108例冠脉造影结果阳性(至少1支主要冠脉内径狭窄≥50.00%)住院患者为观察组,同期住院冠脉造影结果正常者112例作为对照组,通过聚合酶链反应(PCR)和基因测序方法,对EPHX2基因rs751141位点的基因型频率进行对比分析。结果在EPHX2基因rs751141位点上可检测到3种基因型,冠心病组EPHX2基因rs751141位点的基因型频率及C等位基因频率与对照组相比差异有统计学意义(P<0.05)。结论 EPHX2基因rs751141位点SNP和冠心病发病有相关性,携带C等位基因可能是冠心病发病的易感因素之一。
Objective To study the correlation between EPHX2 gene rs751141 polymorphisms and coro- nary heart disease (CHD) in Chinese population. Methods Totally 108 patients having more than one major coronary vessel with at least 50% stenosis defined by coronary angiography were selected as CHD group and control group consisted of 112 healthy subjects. Rs751141 polymorphisms were detected by PCR and gene sequence. Results Three kinds of genotypes at the rs751141 were detected, and no deviation was observed from Hardy- Weinberg equilibrium. There was statistical difference between the two groups regarding the distribution of the geno- type frequencies and the frequency of allele C (P 〈 0.05 ). Conclusion It suggests that rs751141 polymorphism is associated with CHD in Chinese population and the C allele might be a risk factor of CHD.
出处
《实用医学杂志》
CAS
北大核心
2017年第22期3777-3780,共4页
The Journal of Practical Medicine
基金
浙江省温州市科技计划项目(编号:Y20150187)