胭脂萝卜花青素提取及对NCI-N87细胞增殖侵袭的影响

The Effect on NCI-N87 Cell Proliferation and Invasion by Carmine Radish Anthocyanin

为研究胭脂萝卜花青素的提取工艺,考察了液料比、浸提温度、浸提时间、超声功率、超声时间对花青素提取率的影响。采用MTT实验、细胞侵袭实验研究了花青素对人胃癌细胞NCIN87增殖、侵袭的作用,进一步用免疫印迹分析其对HER2信号通过的影响。结果表明,结合响应面分析得出胭脂萝卜花青素最佳提取工艺为:以1%盐酸乙醇为提取剂,液料比10∶1,40℃浸提2h,400W超声波破碎15min,在此条件下,花青素的最高提取量为3.92mg/g。30μg/ml的胭脂萝卜花青素能显著抑制NCI-N87细胞的增殖和侵袭能力,同时抑制HER2蛋白及Akt的磷酸化水平。

To explore the extraction technology of anthocyanin from carmine radish, five single factors were studied including solid-liquid ratio, digestion temperature, digestion time, ultrasonic power, and ultrasonic power. MTT and TranswellTM were used to discuss the proliferation, invasion ability of human gastric carcinoma cells （NCI-N87）which effect by anthocyanin from carmine radish. Furthermore, Western blot used to analyze the HER2 pathway. The optimum extraction technology was determined based on response surface analysis： 1% acidethanol as extracting agent, 1：10 solid-liquid ratio, digestion 2h at 40℃ with 400W ultrasonic disruption for 15min. Under this condition, anthocyanin extraction content was 3.92mg/g. 30p, g/ml anthocyanin from carmine radish inhibited NCI-N87 cell proliferating and invasion ability, decreased HER and p-Akt expression.