通过不同荧光现象识别Cu^(2+)和Zn^(2+)的双吡唑啉荧光探针

Bispyrazoline Fluorescent Probe for the Recognition of Cu^(2+) and Zn^(2+) with Different Fluorescence

设计合成了一个基于双吡唑啉结构的双功能荧光探针1,1'-(5,5'-(6,6'-二羟基-5,5'-二甲氧基联苯-3,3'-二基)双(3-(2-羟基苯基)-1H-吡唑-5,1-(4H,5H)-二基))二乙酮(C1).利用核磁共振氢谱、碳谱、质谱及元素分析对其结构进行了表征,并通过紫外吸收光谱和荧光光谱研究了探针C1及其对金属离子在4-羟乙基哌嗪乙磺酸(HEPES)缓冲溶液[V(MeOH)∶V(H_2O)=1∶1,20 mol·L^(-1),pH=7.4]中的选择性识别作用.结果表明,探针C1对Cu^(2+)和Zn^(2+)有着较高的选择性和灵敏度,并能通过不同的荧光现象对目标离子的识别加以区分.

A bifunctional fluorescent probe 1,1＇-（5,5＇-（6,6＇-dihydroxy-5,5＇-dimethoxybiphenyl-3,3＇-diyl）bis（3-（2-hydroxyphenyl）-1H-pyrazole-5,1（4H,5H）-diyl））diethanone（C1）based on bispyrazoline was designed and synthesized.The structure of C1 was confirmed by ~1H NMR,^（13）C NMR,MS and elemental analysis.The spectroscopic behavior toward metal ions has been investigated by the absorption and fluorescence methods.It exhibited high sensitivity and selectivity for detecting Cu^（2＋）and Zn^（2＋）over other commonly coexistent metal ions in 4-（2-hydroxyethyl）-1-piperazine ethanesulfonic acid（HEPES）buffer solution[V（MeOH）∶V（H_2O）=1∶1,20 mol·L^（-1）,pH=7.4]and it has the capability to differentiate target ions with different fluorescence.