黄芪总皂苷对X线诱导的心肌成纤维细胞纤维化损伤的影响

Effect of Astragaloside on cardiac fibroblasts fibrosis damage induced by X ray

目的观察黄芪总皂苷(AST)对X线照射诱导的心肌成纤维细胞(CFs)纤维化损伤的影响。方法利用小剂量(0.0,0.5,1.0,2.0,3.0,4.0 Gy)X线照射CFs,通过MTT检测细胞增殖活性的改变,通过PCR和Western印迹检测转化生长因子(TGF)-β1和Ⅰ型胶原(Col-Ⅰ)表达水平。结果 1.0 Gy X线照射CFs后48 h作为X线诱导CFs促纤维化损伤的建模条件。AST可剂量依赖性地抑制X线照射CFs产生的活性氧(ROS),降低Col-Ⅰ和TGF-β1、p-Smad2/3 mRNA和蛋白表达水平。结论 AST显著抑制X线诱导的CFs纤维化损伤效应,这种作用可能与其抗ROS作用有关。

Objective To observe the effect of Astragaloside（ AST） on cardiac fibroblasts（ CFs） fibrosis damage induced by X ray.Methods CFs were radiated by 0. 0,0. 5,1. 0,2. 0,3. 0,4. 0 Gy X gray. MTT was used to detect cell proliferation,PCR and Western blot were used to detect transforming growth factor（ TGF）-β1 and Col-Ⅰ expressions.Results Model of CFs fibrosis damage induced by X ray was made by 1. 0 Gy X ray for 48 h. AST could dose dependently inhibite reactive oxygen species（ ROS） produced by CFs damaged by X ray,reduce expressions of Col-Ⅰ,TGF-β1,p-Smads 2/3 mRNA and protein.Conclusions AST could inhibit CFs fibrosis damage induced by X ray,which might be related with its anti-ROS effect.