胸腹水细胞块技术的临床应用研究

Clinical application study of the cell blocks from hydrothorax and ascite

目的探讨石蜡包埋细胞块技术在胸腔积液和腹水（胸腹水）肿瘤细胞诊断中的应用价值。方法对所有临床送检的胸腹水标本进行液基薄层细胞学检查，可疑肿瘤细胞的沉积物制成石蜡包埋细胞块，进行免疫组织化学染色，判断细胞的良恶性，确定肿瘤细胞类型和组织起源。结果对液基薄层细胞学检查诊断为异形细胞、可疑恶性肿瘤或恶性肿瘤的病例69例，进行制作细胞块并结合免疫组织化学染色，确定恶性51例，良性17例，难于确诊1例；恶性确定来源的46例，腺癌不能确定来源的6例。结论石蜡包埋细胞块技术对判断胸腹水中细胞的良恶性、恶性肿瘤的组织来源具有重要价值。

Objective To explore the application value of paraffin embedding cell blocks from hydrothorax and ascite in diagnosis of tumors in the chest. Methods Of all clinical inspection for hydrothorax samples of thin - layer cytology, suspicious made paraffin embedding sediments of the tumor cells, immunohistochemical staining, identified the benign and malignant cells, determined the types of tumor cells and tissue origin. Results For liquid thin -layer cytology diagnosis of heterocyst, suspicious cases of malignant tumor, 69 cases made the cell block combined with immunohistochemical staining, determined the malignant 51 cases, 17 cases were benign, difficult to diagnose in 1 case, malignant determined the source of 46 cases, adenocarcinoma did not determine the source of 6 cases. Conclusion The technique of the embedding of paraffin embedding cells is of great value to the organization of the malignant and malignant tumors of the cells from hydrothorax and ascite.